論文 - 詳細
| RRC ID | 55355 |
|---|---|
| 著者 | Yu Y, Fu Y, Guo X, Yan R, Wang H, Zhao J, Wang X, Zhang J, Xiang W. |
| タイトル | Streptomyces durbertensis sp. nov., isolated from saline-alkali soil. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel strain of actinobacteria, designated NEAU-S1GS20T, was isolated from a saline-alkali soil collected from Heilongjiang Province, north-east China, and characterized using a polyphasic approach. Strain NEAU-S1GS20T exhibited morphological, cultural and chemotaxonomic features consistent with its classification as representing a member of the genus Streptomyces. Growth occurred at 18‒45 °C, at pH 6.0‒10.0 and in the presence of 10 % (w/v) NaCl. Whole-cell hydrolysates mainly contained glucose and ribose. The predominant menaquinones were MK-9(H4) and MK-9(H6). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unidentified phospholipids. The major cellular fatty acids (>10 %) were iso-C16 : 0, iso-C17 : 0, anteiso-C17 : 0 and C16 : 0. The G+C content of the DNA was 72.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain NEAU-S1GS20T formed a distinct clade within the genus Streptomyces and was closely related to Streptomyces xinghaiensis CCTCC AA 208049T (98.4 % similarity), Streptomyces chumphonensis JCM 18522T (98.1 %) and Streptomyces palmae JCM 31289T (98.1 %). Multilocus sequence analysis (MLSA) using five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA distance of strain NEAU-S1GS20T to the most closely related species was greater than the 0.007 threshold. A combination of DNA-DNA hybridization results and differences in certain phenotypic characteristics demonstrated that strain NEAU-S1GS20T could be distinguished from its closest phylogenetic relatives. Therefore, strain NEAU-S1GS20T represents a novel species of the genus Streptomyces, for which the name Streptomyces durbertensis sp. nov. is proposed. The type strain is NEAU-S1GS20T (=CCTCC AA 2017006T=DSM 104538T). |
| 巻・号 | 68(11) |
| ページ | 3635-3640 |
| 公開日 | 2018-11-1 |
| DOI | 10.1099/ijsem.0.003047 |
| PMID | 30303472 |
| MeSH | Alkalies Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids / chemistry Genes, Bacterial Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Salinity Sequence Analysis, DNA Soil / chemistry Soil Microbiology* Streptomyces / classification* Streptomyces / genetics Streptomyces / isolation & purification Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| IF | 2.166 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 31289 JCM 18522 |