| RRC ID |
553
|
| 著者 |
Yamanaka N, Hua YJ, Mizoguchi A, Watanabe K, Niwa R, Tanaka Y, Kataoka H.
|
| タイトル |
Identification of a novel prothoracicostatic hormone and its receptor in the silkworm Bombyx mori.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
The insect brain regulates the activity of the prothoracic glands to secrete ecdysteroids, which affect growth, molting, and metamorphosis. Here we report the identification of a novel prothoracicostatic factor and its receptor in the silkworm Bombyx mori. The prothoracicostatic factor purified from pupal brains of B. mori is a decapeptide with the conserved structure of an insect myosuppressin and thus named Bommo-myosuppressin. Bommo-myosuppressin dose dependently suppressed the cAMP level and inhibited ecdysteroidogenesis in the larval prothoracic glands at much lower concentrations than the prothoracicostatic peptide, the other prothoracicostatic factor reported previously. In vitro analyses using a prothoracic gland incubation method revealed that Bommo-myosuppressin and prothoracicostatic peptide regulate the prothoracic gland activity via different receptors. In situ hybridization and immunohistochemistry revealed the existence of Bommo-myosuppressin in the brain neurosecretory cells projecting to neurohemal organs in which it is stored. We also identified and functionally characterized a specific receptor for Bommo-myosuppressin and showed its high expression in the prothoracic glands. All these results suggest that Bommo-myosuppressin functions as a prothoracicostatic hormone and plays an important role in controlling insect development.
|
| 巻・号 |
280(15)
|
| ページ |
14684-90
|
| 公開日 |
2005-4-15
|
| DOI |
10.1074/jbc.M500308200
|
| PII |
S0021-9258(20)65988-9
|
| PMID |
15701625
|
| MeSH |
Amino Acid Sequence
Animals
Blotting, Northern
Bombyx
Brain / metabolism
Calcium / metabolism
Cell Line
Chromatography, High Pressure Liquid
Cyclic AMP / metabolism
DNA, Complementary / metabolism
Databases as Topic
Ecdysone / chemistry
Expressed Sequence Tags
Gene Library
Humans
Image Processing, Computer-Assisted
Immunohistochemistry
In Situ Hybridization
Insect Hormones / biosynthesis*
Insect Hormones / chemistry*
Ligands
Mass Spectrometry
Metamorphosis, Biological
Molecular Sequence Data
Neuropeptides / chemistry*
Neuropeptides / physiology*
Peptides / chemistry
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry
Ultraviolet Rays
|
| IF |
4.238
|
| 引用数 |
63
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
EST database |
