RRC ID 55425
Author Lam KL, Lin S, Liu C, Wu X, Tang S, Kwan HS, Cheung PC.
Title Low-Cost Method Generating In Situ Anaerobic Conditions on a 96-Well Plate for Microbial Fermentation in Food Research.
Journal J Agric Food Chem
Abstract Commercial tools and instruments have been developed for a screening study of microbial fermentation, but they are expensive and mostly confined to aerobic fermentation only. There is little development on the generation of anaerobic conditions directly on a 96-well plate. This report proposed a simple and versatile microbial fermentation system known as OVAMO that makes use of Oxyrase, vacuum, and mineral oil to generate an in situ anaerobic environment on a 96-well plate for at least 48 h. The practicality of OVAMO in anaerobic fermentation experiments used for functional food research was validated by a prebiotic screening study of different carbohydrates by Bifidobacterium longum subsp. infantis. The OVAMO system provides a less expensive but effective way to conduct a microbial fermentation screening study that requires anaerobic conditions without the need for atmospheric control by external devices.
Volume 66(44)
Pages 11839-11845
Published 2018-11-7
DOI 10.1021/acs.jafc.8b04888
PMID 30277075
MeSH Anaerobiosis Bifidobacterium / growth & development Bifidobacterium / metabolism* Fermentation Food Microbiology / economics Food Microbiology / instrumentation Food Microbiology / methods* Oxygen / analysis Oxygen / metabolism Prebiotics / analysis
IF 3.571
Times Cited 1
General Microbes JCM 1222