論文 - 詳細
| RRC ID | 55425 |
|---|---|
| 著者 | Lam KL, Lin S, Liu C, Wu X, Tang S, Kwan HS, Cheung PC. |
| タイトル | Low-Cost Method Generating In Situ Anaerobic Conditions on a 96-Well Plate for Microbial Fermentation in Food Research. |
| ジャーナル | J Agric Food Chem |
| Abstract |
Commercial tools and instruments have been developed for a screening study of microbial fermentation, but they are expensive and mostly confined to aerobic fermentation only. There is little development on the generation of anaerobic conditions directly on a 96-well plate. This report proposed a simple and versatile microbial fermentation system known as OVAMO that makes use of Oxyrase, vacuum, and mineral oil to generate an in situ anaerobic environment on a 96-well plate for at least 48 h. The practicality of OVAMO in anaerobic fermentation experiments used for functional food research was validated by a prebiotic screening study of different carbohydrates by Bifidobacterium longum subsp. infantis. The OVAMO system provides a less expensive but effective way to conduct a microbial fermentation screening study that requires anaerobic conditions without the need for atmospheric control by external devices. |
| 巻・号 | 66(44) |
| ページ | 11839-11845 |
| 公開日 | 2018-11-7 |
| DOI | 10.1021/acs.jafc.8b04888 |
| PMID | 30277075 |
| MeSH | Anaerobiosis Bifidobacterium / growth & development Bifidobacterium / metabolism* Fermentation Food Microbiology / economics Food Microbiology / instrumentation Food Microbiology / methods* Oxygen / analysis Oxygen / metabolism Prebiotics / analysis |
| IF | 3.571 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1222 |