論文 - 詳細
| RRC ID | 55426 |
|---|---|
| 著者 | Suzuki H, Taketani T, Kobayashi J, Ohshiro T. |
| タイトル | Antibiotic resistance mutations induced in growing cells of Bacillus-related thermophiles. |
| ジャーナル | J Antibiot (Tokyo) |
| Abstract |
Stress-induced mutagenesis can assist pathogens in generating drug-resistant cells during antibiotic therapy; however, if and how antibiotics induce mutagenesis in microbes remains poorly understood. A non-pathogenic thermophile, Geobacillus kaustophilus HTA426, efficiently produces derivative cells resistant to rifampicin and streptomycin via rpoB and rpsL mutations, respectively. Here, we examined this phenomenon to suggest a novel mutagenic mode induced by antibiotics. Fluctuation analysis indicated that mutations occurred via spontaneous mutations during culture. However, mutations were much more frequent in growing cells than stationary cells, and mutation sites were varied through cell growth. These observations suggested that growing cells induced mutagenesis in response to antibiotics. An in-frame deletion of mfd, which governs transcription-coupled repair to correct DNA lesions on the transcribed strand, caused mutations that were comparable between growing and stationary cells; therefore, the mutagenic mechanism was attributable to DNA repair defects where growing cells depressed mfd function. Mutations occurred more frequently at optimal growth temperatures for G. kaustophilus than at a higher growth temperature, suggesting that the mutagenesis relies on active cellular activities rather than high temperature-associated DNA damage. In addition, the mutagenesis may involve a mutagenic factor targeting these sites, in addition to mfd depression, because rpoB and rpsL mutations were dominant at thymine and guanine sites on the transcribed strand. A similar mutagenic profile was observed for other Geobacillus and thermophilic Bacillus species. This suggests that Bacillus-related thermophiles commonly induce mutagenesis in response to rifampicin and streptomycin to produce resistant cells. |
| 巻・号 | 71(3) |
| ページ | 382-389 |
| 公開日 | 2018-3-1 |
| DOI | 10.1038/s41429-017-0003-1 |
| PII | 10.1038/s41429-017-0003-1 |
| PMID | 29348523 |
| MeSH | Anti-Bacterial Agents / pharmacology* Bacillus / drug effects* Bacillus / genetics* DNA Repair / drug effects Drug Resistance, Bacterial / genetics* Geobacillus / drug effects Mutagenesis Mutation / genetics Temperature |
| IF | 2.446 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 12893 |