論文 - 詳細
| RRC ID | 55470 |
|---|---|
| 著者 | Hasegawa S, Jojima T, Inui M. |
| タイトル | Efficient construction of xenogeneic genomic libraries by circumventing restriction-modification systems that restrict methylated DNA. |
| ジャーナル | J Microbiol Methods |
| Abstract |
An efficient method to construct xenogeneic genomic libraries with low errors and bias by circumventing restriction-modification systems that restrict methylated DNA was developed. Un-methylated genomic DNA of Escherichia coli prepared by ϕ29 DNA polymerase was introduced to Corynebacterium glutamicum R after ligation with un-methylated vector plasmids. |
| 巻・号 | 146 |
| ページ | 13-15 |
| 公開日 | 2018-3-1 |
| DOI | 10.1016/j.mimet.2018.01.005 |
| PII | S0167-7012(18)30009-5 |
| PMID | 29355574 |
| MeSH | Cloning, Molecular / methods* Corynebacterium glutamicum / genetics DNA Methylation* DNA Restriction-Modification Enzymes* DNA, Bacterial DNA-Directed DNA Polymerase Escherichia coli / genetics Genes, Bacterial / genetics Genetic Vectors / genetics Genomic Library* Plasmids Transformation, Bacterial |
| IF | 1.701 |
| 引用数 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 18229 |