論文 - 詳細
| RRC ID | 55511 |
|---|---|
| 著者 | Ochiai A, Ogawa K, Fukuda M, Ohori M, Kanaoka T, Tanaka T, Taniguchi M, Sagehashi Y. |
| タイトル | Rice Defensin OsAFP1 is a New Drug Candidate against Human Pathogenic Fungi. |
| ジャーナル | Sci Rep |
| Abstract |
Fungal infections, such as candidiasis and aspergillosis, are some of the most frequent infections in humans. Although antifungal drugs are available for the treatment of these infections, antifungal agents with new mechanisms of action should be developed because of the increasing incidence of drug-resistant pathogens in recent years. In this study, a basic functional analysis of rice defensin OsAFP1, a novel antifungal drug candidate, was conducted. OsAFP1 exerted fungicidal activity against Candida albicans, the most common pathogenic fungus in humans, at 4 μM concentration, but it did not inhibit the growth of human pathogenic bacteria. In addition, OsAFP1 retained structural stability after heat treatment at 100 °C for 10 min and after serum treatment at 37 °C for 24 h. A propidium iodide (PI) uptake assay and mutational analysis revealed that amino acid residues within the C-terminal γ-core motif of OsAFP1, particularly Leu-39 and Lys-41, play an important role in its antifungal activity. Further, PI uptake and apoptosis assays suggested that OsAFP1 exerts its antifungal activity by inducing apoptosis of target cells. Immunohistochemistry showed that the OsAFP1 target molecule was located in the cell wall. These findings indicate that OsAFP1 may be developed into a potent antifungal drug. |
| 巻・号 | 8(1) |
| ページ | 11434 |
| 公開日 | 2018-7-30 |
| DOI | 10.1038/s41598-018-29715-w |
| PII | 10.1038/s41598-018-29715-w |
| PMID | 30061724 |
| PMC | PMC6065317 |
| MeSH | Amino Acid Sequence Antifungal Agents / chemistry Antifungal Agents / pharmacology* Apoptosis / drug effects Candida albicans / cytology Candida albicans / drug effects* Cell Membrane / drug effects Cell Membrane / metabolism Cell Wall / drug effects Cell Wall / metabolism Defensins / chemistry Defensins / pharmacology* Humans Microbial Sensitivity Tests Mutation / genetics Oryza / chemistry* Plant Proteins / chemistry Plant Proteins / pharmacology* Protein Stability Serum Structural Homology, Protein Temperature |
| IF | 4.011 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 5705 JCM 6473 |