論文 - 詳細
| RRC ID | 5568 |
|---|---|
| 著者 | Kim JM, Ogura A. |
| タイトル | Changes in allele-specific association of histone modifications at the imprinting control regions during mouse preimplantation development. |
| ジャーナル | Genesis |
| Abstract |
Allele-specific association of histone modification is observed at the regulatory region of imprinted genes and has been suggested to work as an epigenetic marker for monoallelic gene expression, along with the allelic CpG methylation of DNA. Although the parent-origin-specific epigenetic status in imprinted genes is thought to be established during preimplantation development, little is known about the allelic specificity of histone modifications during this period because of the limited volume of material available for analysis. In this study, we first revealed the allelic enrichment of histone modifications and variant histones at the imprinting control regions (ICRs) of four-cell to blastocyst stage preimplantation embryos by using carrier chromatin immunoprecipitation and sequence polymorphism analysis of immunoprecipitated DNA. We found relative enrichment of histone H3 lysine 9 dimethylation at the imprinted alleles of ICRs and obtained the results suggesting that histone modifications at ICRs are established during a late preimplantation stage. |
| 巻・号 | 47(9) |
| ページ | 611-6 |
| 公開日 | 2009-9-1 |
| DOI | 10.1002/dvg.20541 |
| PMID | 19530139 |
| MeSH | Alleles* Animals Blastocyst / physiology* Chromatin Immunoprecipitation DNA Primers / genetics Embryonic Development / physiology* Epigenesis, Genetic / genetics* Female Genomic Imprinting / genetics* Genomic Imprinting / physiology Histones / genetics* Histones / metabolism Immunoprecipitation Mice Mice, Inbred C57BL Polymorphism, Single-Stranded Conformational |
| IF | 1.76 |
| 引用数 | 12 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| 実験動物マウス | RBRC00639 |