| RRC ID |
55691
|
| 著者 |
Li C, Haslam TM, Krüger A, Schneider LM, Mishina K, Samuels L, Yang H, Kunst L, Schaffrath U, Nawrath C, Chen G, Komatsuda T, von Wettstein-Knowles P.
|
| タイトル |
The β-Ketoacyl-CoA Synthase HvKCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew.
|
| ジャーナル |
Plant Cell Physiol
|
| Abstract |
The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH was deduced from wax and cutin analyses and by heterologous expression in yeast. Combined, these experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C16-C20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis. The contribution of CER-ZH to water barrier properties of the cuticle and its influence on the germination of barley powdery mildew fungus were also assessed.
|
| 巻・号 |
59(4)
|
| ページ |
806-822
|
| 公開日 |
2018-4-1
|
| DOI |
10.1093/pcp/pcy020
|
| PII |
4834007
|
| PMID |
29401261
|
| MeSH |
3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / metabolism*
Ascomycota / growth & development*
Chromosome Mapping
Conserved Sequence
Crystallography, X-Ray
Dehydration
Droughts
Fatty Acids / metabolism
Gene Expression Regulation, Plant
Genes, Plant
Genetic Association Studies
Hordeum / enzymology*
Hordeum / genetics
Membrane Lipids / metabolism
Mutation / genetics
Phenotype
Plant Diseases / microbiology*
Plant Leaves / metabolism*
Plant Proteins / metabolism*
Saccharomyces cerevisiae / metabolism
Stress, Physiological / genetics
Transcription, Genetic
Waxes / metabolism*
|
| IF |
3.929
|
| 引用数 |
6
|
| リソース情報 |
| オオムギ |
Seed sample of H602 |
