RRC ID 55691
Author Li C, Haslam TM, Krüger A, Schneider LM, Mishina K, Samuels L, Yang H, Kunst L, Schaffrath U, Nawrath C, Chen G, Komatsuda T, von Wettstein-Knowles P.
Title The β-Ketoacyl-CoA Synthase HvKCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew.
Journal Plant Cell Physiol.
Abstract The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH was deduced from wax and cutin analyses and by heterologous expression in yeast. Combined, these experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C16-C20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis. The contribution of CER-ZH to water barrier properties of the cuticle and its influence on the germination of barley powdery mildew fungus were also assessed.
Volume 59(4)
Pages 806-822
Published 2018-4-1
DOI 10.1093/pcp/pcy020
PII 4834007
PMID 29401261
MeSH 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / metabolism* Ascomycota / growth & development* Chromosome Mapping Conserved Sequence Crystallography, X-Ray Dehydration Droughts Fatty Acids / metabolism Gene Expression Regulation, Plant Genes, Plant Genetic Association Studies Hordeum / enzymology* Hordeum / genetics Membrane Lipids / metabolism Mutation / genetics Phenotype Plant Diseases / microbiology* Plant Leaves / metabolism* Plant Proteins / metabolism* Saccharomyces cerevisiae / metabolism Stress, Physiological / genetics Transcription, Genetic Waxes / metabolism*
IF 3.929
Times Cited 6
Resource
Barley Seed sample of H602