論文 - 詳細
| RRC ID | 55707 |
|---|---|
| 著者 | Nagasawa K, Ishida M, Octavera A, Kusano K, Kezuka F, Kitano T, Yoshiura Y, Yoshizaki G. |
| タイトル | Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation†. |
| ジャーナル | Biol Reprod |
| Abstract |
A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via spermatogonial transplantation (SGTP). We previously discovered that female medaka carrying mutations on the follicle-stimulating hormone receptor (fshr) gene become sterile. In this study, we demonstrated that sterile hybrid recipient females that received spermatogonia isolated from sex-reversed XX males (fshr (-/-)) recovered their fertility and produced only donor-derived fshr (-) X eggs. Natural mating between these females and fshr (-/-) sex-reversed XX males successfully produced large numbers of sterile fshr (-/-) female offspring. In conclusion, we established a new strategy for efficient mass production of sterile fish. This system can be applied to any aquaculture species for which SGTP and methods for producing sterile recipients can be established. |
| 巻・号 | 100(2) |
| ページ | 535-546 |
| 公開日 | 2019-2-1 |
| DOI | 10.1093/biolre/ioy204 |
| PII | 5106316 |
| PMID | 30252024 |
| MeSH | Animals Aromatase Inhibitors / pharmacology Female Gene Deletion Gene Expression Regulation / drug effects Gene Expression Regulation / genetics Genotype Hybridization, Genetic Infertility / genetics Infertility / veterinary* Male Oryzias / genetics* Oryzias / physiology* Receptors, FSH / genetics* Spermatogonia / physiology* |
| IF | 2.96 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | hi-medaka (MT835) |