RRC ID 5577
著者 Hatanaka Y, Matsumoto T, Yanagawa Y, Fujisawa H, Murakami F, Masu M.
タイトル Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
ジャーナル J Comp Neurol
Abstract Cortical excitatory neurons migrate from their origin in the ventricular zone (VZ) toward the pial surface. During migration, these neurons exhibit a stellate shape in the intermediate zone (IZ), transform into bipolar cells, and then initiate radial migration, extending a trailing process, which may lead to an axon. Here we examined the role of neuropilin 1 (NRP1) in these developmental events. Both NRP1 mRNA and protein were highly expressed in the IZ, where stellate-shaped cells were located. DiI labeling experiments showed that neuronal migration occurred normally in Nrp1 mutant mice up to embryonic day (E) 14.5, the latest day to which the mutant survives, with only subtle axonal defasciculation. However, interference with Nrp1 signaling at a later stage caused pathfinding errors: when a dominant negative form of Nrp1 was electroporated into the cortical VZ cells at E12.5 or E15.5 and examined perinatally, guidance errors were found in tangential axonal extension toward the midline. In contrast, no significant effect was noted on the migration of cortical excitatory neurons. These findings indicate that NRP1 plays an important role in the guidance of callosal axons originating from cortical excitatory neurons but does not support a role in their migration. Moreover, insofar as radial axonal extension within the cortical plate was unaffected, the present findings imply that molecular mechanisms for the axonal extension of excitatory neurons within the cortical plate are distinct from those in the white matter.
巻・号 514(3)
ページ 215-25
公開日 2009-5-20
DOI 10.1002/cne.22021
PMID 19296474
MeSH Animals Axons / physiology* Axons / ultrastructure Cell Movement* Cells, Cultured Cerebral Cortex / cytology Cerebral Cortex / embryology Cerebral Cortex / physiology* Corpus Callosum / cytology* Electroporation Immunohistochemistry In Situ Hybridization Mice Mice, Inbred ICR Mice, Transgenic Neurons / cytology Neurons / physiology* Neuropilin-1 / genetics Neuropilin-1 / metabolism* RNA, Messenger / metabolism
IF 2.801
引用数 23
WOS 分野 ZOOLOGY NEUROSCIENCES
リソース情報
実験動物マウス RBRC01058