論文 - 詳細
| RRC ID | 55785 |
|---|---|
| 著者 | Nishishita N, Ijiri H, Takenaka C, Kobayashi K, Goto K, Kotani E, Itoh T, Mori H, Kawamata S. |
| タイトル | The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells. |
| ジャーナル | Biomaterials |
| Abstract |
Human leukemia inhibitory factor (LIF) was immobilized into insect virus-derived microcrystals (polyhedra) to generate LIF polyhedra (LIF-PH) that can slowly release LIF into embryonic stem (ES) cell culture media and thus maintain ES cells in an undifferentiated state. Assays of the biological activities of LIF-PH indicated that a single addition of LIF-PH to the ES cell culture medium can support the proliferation of mouse ES and induced pluripotent stem (iPS) cells continuously for 14 days, and suggest that LIF-PH can be successfully used in the place of a periodic addition of recombinant LIF to the media every 2-3 days. The release of LIF protein from LIF-PH was determined by enzyme-linked immunosorbent assay (ELISA). Maintenance of undifferentiated state of mouse ES and iPS cells cultured with LIF-PH was determined by the detection of pluripotency-related biomarkers Oct3/4 and stage-specific embryonic antigen-1 (SSEA-1) through immunostaining and measurement of alkaline phosphatase activity. In this paper, we propose a closed culture system for mass production of ES and iPS cells that utilize a slow-releasing agent of LIF. |
| 巻・号 | 32(14) |
| ページ | 3555-63 |
| 公開日 | 2011-5-1 |
| DOI | 10.1016/j.biomaterials.2010.12.063 |
| PII | S0142-9612(11)00086-X |
| PMID | 21315447 |
| MeSH | Animals Blotting, Western Cell Differentiation / drug effects Cell Line Cell Proliferation / drug effects Embryonic Stem Cells / cytology* Embryonic Stem Cells / drug effects* Enzyme-Linked Immunosorbent Assay Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects* Leukemia Inhibitory Factor / chemistry Leukemia Inhibitory Factor / pharmacology* Mice STAT3 Transcription Factor / metabolism |
| IF | 10.273 |
| 引用数 | 18 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 研究用ヒト臍帯血幹細胞 | |