RRC ID 55785
Author Nishishita N, Ijiri H, Takenaka C, Kobayashi K, Goto K, Kotani E, Itoh T, Mori H, Kawamata S.
Title The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells.
Journal Biomaterials
Abstract Human leukemia inhibitory factor (LIF) was immobilized into insect virus-derived microcrystals (polyhedra) to generate LIF polyhedra (LIF-PH) that can slowly release LIF into embryonic stem (ES) cell culture media and thus maintain ES cells in an undifferentiated state. Assays of the biological activities of LIF-PH indicated that a single addition of LIF-PH to the ES cell culture medium can support the proliferation of mouse ES and induced pluripotent stem (iPS) cells continuously for 14 days, and suggest that LIF-PH can be successfully used in the place of a periodic addition of recombinant LIF to the media every 2-3 days. The release of LIF protein from LIF-PH was determined by enzyme-linked immunosorbent assay (ELISA). Maintenance of undifferentiated state of mouse ES and iPS cells cultured with LIF-PH was determined by the detection of pluripotency-related biomarkers Oct3/4 and stage-specific embryonic antigen-1 (SSEA-1) through immunostaining and measurement of alkaline phosphatase activity. In this paper, we propose a closed culture system for mass production of ES and iPS cells that utilize a slow-releasing agent of LIF.
Volume 32(14)
Pages 3555-63
Published 2011-5
DOI 10.1016/j.biomaterials.2010.12.063
PII S0142-9612(11)00086-X
PMID 21315447
MeSH Animals Blotting, Western Cell Differentiation / drug effects Cell Line Cell Proliferation / drug effects Embryonic Stem Cells / cytology* Embryonic Stem Cells / drug effects* Enzyme-Linked Immunosorbent Assay Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects* Leukemia Inhibitory Factor / chemistry Leukemia Inhibitory Factor / pharmacology* Mice STAT3 Transcription Factor / metabolism
IF 10.273
Resource
Cord blood stem cells for research