RRC ID 55831
Author Moukadiri I, Villarroya M, Benítez-Páez A, Armengod ME.
Title Bacillus subtilis exhibits MnmC-like tRNA modification activities.
Journal RNA Biol
Abstract The MnmE-MnmG complex of Escherichia coli uses either ammonium or glycine as a substrate to incorporate the 5-aminomethyl or 5-carboxymethylaminomethyl group into the wobble uridine of certain tRNAs. Both modifications can be converted into a 5-methylaminomethyl group by the independent oxidoreductase and methyltransferase activities of MnmC, which respectively reside in the MnmC(o) and MnmC(m) domains of this bifunctional enzyme. MnmE and MnmG, but not MnmC, are evolutionarily conserved. Bacillus subtilis lacks genes encoding MnmC(o) and/or MnmC(m) homologs. The glycine pathway has been considered predominant in this typical gram-positive species because only the 5-carboxymethylaminomethyl group has been detected in tRNALysUUU and bulk tRNA to date. Here, we show that the 5-methylaminomethyl modification is prevalent in B. subtilis tRNAGlnUUG and tRNAGluUUC. Our data indicate that B. subtilis has evolved MnmC(o)- and MnmC(m)-like activities that reside in non MnmC homologous protein(s), which suggests that both activities provide some sort of biological advantage.
Volume 15(9)
Pages 1167-1173
Published 2018-9-24
DOI 10.1080/15476286.2018.1517012
PMID 30249152
PMC PMC6284559
MeSH Bacillus subtilis / genetics Bacterial Proteins / genetics Bacterial Proteins / metabolism Escherichia coli Proteins / metabolism Multienzyme Complexes / metabolism Mutation One-Carbon Group Transferases / genetics One-Carbon Group Transferases / metabolism RNA Processing, Post-Transcriptional RNA, Transfer, Gln / metabolism* RNA, Transfer, Glu / metabolism* Uridine / metabolism*
IF 5.477
Resource
Prokaryotes B. subtilis JW3719 MGNA-A001, MGNA-B876, MGNA-B877, MGNA-B901, MGNA-C276, MGNA-A931, MGNA-B438, MGNA-B690, MGNA-C085, MGNA-C051, MGNA-C499, MGNA-A165, MGNA-A880, MGNA-C369, MGNA-A114, MGNA-B147, MGNA-B195, MGNA-B222, MGNA-B368, MGNA-B726, MGNA-B731, MGNA-B732, MGNA-B875, MGNA-B884, MGNA-B903, MGNA-B941, MGNA-B946, MGNA-C050, MGNA-C428, MGNA-C496, MGNA-C498, MGNA-C052