| RRC ID |
55831
|
| Author |
Moukadiri I, Villarroya M, Benítez-Páez A, Armengod ME.
|
| Title |
Bacillus subtilis exhibits MnmC-like tRNA modification activities.
|
| Journal |
RNA Biol
|
| Abstract |
The MnmE-MnmG complex of Escherichia coli uses either ammonium or glycine as a substrate to incorporate the 5-aminomethyl or 5-carboxymethylaminomethyl group into the wobble uridine of certain tRNAs. Both modifications can be converted into a 5-methylaminomethyl group by the independent oxidoreductase and methyltransferase activities of MnmC, which respectively reside in the MnmC(o) and MnmC(m) domains of this bifunctional enzyme. MnmE and MnmG, but not MnmC, are evolutionarily conserved. Bacillus subtilis lacks genes encoding MnmC(o) and/or MnmC(m) homologs. The glycine pathway has been considered predominant in this typical gram-positive species because only the 5-carboxymethylaminomethyl group has been detected in tRNALysUUU and bulk tRNA to date. Here, we show that the 5-methylaminomethyl modification is prevalent in B. subtilis tRNAGlnUUG and tRNAGluUUC. Our data indicate that B. subtilis has evolved MnmC(o)- and MnmC(m)-like activities that reside in non MnmC homologous protein(s), which suggests that both activities provide some sort of biological advantage.
|
| Volume |
15(9)
|
| Pages |
1167-1173
|
| Published |
2018-9-24
|
| DOI |
10.1080/15476286.2018.1517012
|
| PMID |
30249152
|
| PMC |
PMC6284559
|
| MeSH |
Bacillus subtilis / genetics
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Escherichia coli Proteins / metabolism
Multienzyme Complexes / metabolism
Mutation
One-Carbon Group Transferases / genetics
One-Carbon Group Transferases / metabolism
RNA Processing, Post-Transcriptional
RNA, Transfer, Gln / metabolism*
RNA, Transfer, Glu / metabolism*
Uridine / metabolism*
|
| IF |
5.477
|
| Times Cited |
2
|
| Resource |
| Prokaryotes B. subtilis |
JW3719
MGNA-A001
MGNA-B876
MGNA-B877
MGNA-B901
MGNA-C276
MGNA-A931
MGNA-B438
MGNA-B690
MGNA-C085
MGNA-C051
MGNA-C499
MGNA-A165
MGNA-A880
MGNA-C369
MGNA-A114
MGNA-B147
MGNA-B195
MGNA-B222
MGNA-B368
MGNA-B726
MGNA-B731
MGNA-B732
MGNA-B875
MGNA-B884
MGNA-B903
MGNA-B941
MGNA-B946
MGNA-C050
MGNA-C428
MGNA-C496
MGNA-C498
MGNA-C052 |
