| RRC ID |
55831
|
| 著者 |
Moukadiri I, Villarroya M, Benítez-Páez A, Armengod ME.
|
| タイトル |
Bacillus subtilis exhibits MnmC-like tRNA modification activities.
|
| ジャーナル |
RNA Biol
|
| Abstract |
The MnmE-MnmG complex of Escherichia coli uses either ammonium or glycine as a substrate to incorporate the 5-aminomethyl or 5-carboxymethylaminomethyl group into the wobble uridine of certain tRNAs. Both modifications can be converted into a 5-methylaminomethyl group by the independent oxidoreductase and methyltransferase activities of MnmC, which respectively reside in the MnmC(o) and MnmC(m) domains of this bifunctional enzyme. MnmE and MnmG, but not MnmC, are evolutionarily conserved. Bacillus subtilis lacks genes encoding MnmC(o) and/or MnmC(m) homologs. The glycine pathway has been considered predominant in this typical gram-positive species because only the 5-carboxymethylaminomethyl group has been detected in tRNALysUUU and bulk tRNA to date. Here, we show that the 5-methylaminomethyl modification is prevalent in B. subtilis tRNAGlnUUG and tRNAGluUUC. Our data indicate that B. subtilis has evolved MnmC(o)- and MnmC(m)-like activities that reside in non MnmC homologous protein(s), which suggests that both activities provide some sort of biological advantage.
|
| 巻・号 |
15(9)
|
| ページ |
1167-1173
|
| 公開日 |
2018-9-24
|
| DOI |
10.1080/15476286.2018.1517012
|
| PMID |
30249152
|
| PMC |
PMC6284559
|
| MeSH |
Bacillus subtilis / genetics
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Escherichia coli Proteins / metabolism
Multienzyme Complexes / metabolism
Mutation
One-Carbon Group Transferases / genetics
One-Carbon Group Transferases / metabolism
RNA Processing, Post-Transcriptional
RNA, Transfer, Gln / metabolism*
RNA, Transfer, Glu / metabolism*
Uridine / metabolism*
|
| IF |
5.477
|
| 引用数 |
2
|
| リソース情報 |
| 原核生物(枯草菌) |
JW3719
MGNA-A001, MGNA-B876, MGNA-B877, MGNA-B901, MGNA-C276, MGNA-A931, MGNA-B438, MGNA-B690, MGNA-C085, MGNA-C051, MGNA-C499, MGNA-A165, MGNA-A880, MGNA-C369, MGNA-A114, MGNA-B147, MGNA-B195, MGNA-B222, MGNA-B368, MGNA-B726, MGNA-B731, MGNA-B732, MGNA-B875, MGNA-B884, MGNA-B903, MGNA-B941, MGNA-B946, MGNA-C050, MGNA-C428, MGNA-C496, MGNA-C498, MGNA-C052 |
