| RRC ID |
55947
|
| 著者 |
Yoshida H, Yamada H, Nogami W, Dohi K, Kurino-Yamada T, Sugiyama K, Takahashi K, Gahara Y, Kitaura M, Hasegawa M, Oshima I, Kuwabara K.
|
| タイトル |
Development of a new knock-in mouse model and evaluation of pharmacological activities of lusutrombopag, a novel, nonpeptidyl small-molecule agonist of the human thrombopoietin receptor c-Mpl.
|
| ジャーナル |
Exp Hematol
|
| Abstract |
Lusutrombopag (S-888711), an oral small-molecule thrombopoietin receptor (TPOR) agonist, has gained first approval as a drug to treat thrombocytopenia of chronic liver disease in patients undergoing elective invasive procedures in Japan. Preclinical studies were performed to evaluate its efficacy against megakaryopoiesis and thrombopoiesis. To investigate the proliferative activity and efficacy of megakaryocytic colony formation via human TPOR, lusutrombopag was applied to cultured human c-Mpl-expressing Ba/F3 (Ba/F3-hMpl) cells and human bone marrow-derived CD34-positive cells, respectively. Lusutrombopag caused a robust increase in Ba/F3-hMpl cells by activating pathways in a manner similar to that of thrombopoietin and induced colony-forming units-megakaryocyte and polyploid megakaryocytes in human CD34-positive cells. Because lusutrombopag has high species specificity for human TPOR, there was no suitable experimental animal model for drug evaluation, except for immunodeficient mouse-based xenograft models. Therefore, a novel genetically modified knock-in mouse, TPOR-Ki/Shi, was developed by replacing mouse Mpl with human-mouse chimera Mpl. In TPOR-Ki/Shi mice, lusutrombopag significantly increased circulating platelets in a dose-dependent manner during 21-day repeated oral administration. Histopathological study of the TPOR-Ki/Shi mice on day 22 also revealed a significant increase in megakaryocytes in the bone marrow. These results indicate that lusutrombopag acts on human TPOR to upregulate differentiation and proliferation of megakaryocytic cells, leading to platelet production.
|
| 巻・号 |
59
|
| ページ |
30-39.e2
|
| 公開日 |
2018-3-1
|
| DOI |
10.1016/j.exphem.2017.12.005
|
| PII |
S0301-472X(17)30904-9
|
| PMID |
29274361
|
| MeSH |
Animals
Blood Platelets / cytology
Blood Platelets / metabolism
Cell Line
Cell Proliferation / drug effects*
Cinnamates / pharmacology*
Drug Evaluation, Preclinical
Gene Knock-In Techniques
Humans
Megakaryocytes / cytology
Megakaryocytes / metabolism*
Mice
Mice, Transgenic
Models, Biological*
Receptors, Thrombopoietin / agonists*
Receptors, Thrombopoietin / genetics
Receptors, Thrombopoietin / metabolism
Thiazoles / pharmacology*
|
| IF |
2.462
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805) |
