| RRC ID |
55957
|
| 著者 |
Mushirobira Y, Nishimiya O, Nagata J, Todo T, Hara A, Reading BJ, Hiramatsu N.
|
| タイトル |
Molecular cloning of vitellogenin gene promoters and in vitro and in vivo transcription profiles following estradiol-17β administration in the cutthroat trout.
|
| ジャーナル |
Gen Comp Endocrinol
|
| Abstract |
Transcription of vitellogenin (vtg) genes are initiated when estradiol-17β (E2)-estrogen receptor (ER) complexes bind estrogen response elements (ERE) located in the gene promoter region. Transcriptional regulation of dual vtg subtypes (major salmonid A-type vtg: vtgAs; minor C-type vtg: vtgC) by E2 was investigated under co-expression of a potential major transcriptional factor, erα1, in cutthroat trout. Two forms of trout vtgAs promoters (1 and 2) and one vtgC promoter were sequenced. These promoters structurally differ based on the number of EREs present. The vtgAs promoter 1 exhibited the highest maximal transcriptional activity by in vitro gene reporter assays. The concentration of E2 that induces 50% of gene reporter activity (half-maximal effective concentrations, EC50) was similar among all vtg promoters and also to the EC50 of E2 administered to induce vtg transcription in vivo. This study revealed a difference in transcriptional properties of multiple vtg promoters for the first time in a salmonid species, providing the basis to understand mechanisms underlying regulation of vitellogenesis via dual vtg gene expression.
|
| 巻・号 |
267
|
| ページ |
157-166
|
| 公開日 |
2018-10-1
|
| DOI |
10.1016/j.ygcen.2018.06.017
|
| PII |
S0016-6480(18)30064-9
|
| PMID |
29966659
|
| MeSH |
Animals
Base Sequence
CHO Cells
Cloning, Molecular
Cricetinae
Cricetulus
Estradiol / administration & dosage*
Estradiol / blood
Estradiol / pharmacology*
Female
Gene Expression Profiling
Gene Expression Regulation / drug effects
Genes, Reporter
Liver / metabolism
Luciferases / metabolism
Oncorhynchus / blood
Oncorhynchus / genetics*
Promoter Regions, Genetic*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Analysis, DNA
Transcription, Genetic / drug effects*
Vitellogenesis / drug effects
Vitellogenesis / genetics
Vitellogenins / genetics*
Vitellogenins / metabolism
|
| IF |
2.445
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
CHO-K1(RCB2330) |
