RRC ID 5603
Author Punnamoottil B, Kikuta H, Pezeron G, Erceg J, Becker TS, Rinkwitz S.
Title Enhancer detection in zebrafish permits the identification of neuronal subtypes that express Hox4 paralogs.
Journal Dev. Dyn.
Abstract Activity of zebrafish hoxb4a in the developing brain was analyzed in comparison to hoxa4a and hoxd4a using unique enhancer detection transgenes. Cytoplasmic YFP revealed shape and axonal projections of neurons in animals with insertions near the Hox4 genes and provided a means for the identification of neuronal subtypes. Despite an early activity of the genes in neuroepithelial cells and later in immature postmitotic neurons, we found reporter expression in distinct neuronal subtypes in the r7-r8-derived hindbrain. Most strikingly, hoxb4a neuronal subtypes projected through the vagus and into the pectoral fin while others formed symmetrically located fiber tracts innervating the cerebellum and the tectum, features that are partially shared by the other two paralogs. Collectively, our expression analysis indicates that hoxb4a in combination with its paralogs may play a significant role in the development of precerebellar, vagal, and pectoral fin neuronal subtypes.
Volume 237(8)
Pages 2195-208
Published 2008-8
DOI 10.1002/dvdy.21618
PMID 18627100
MeSH Animals Branchial Region / cytology Branchial Region / embryology Branchial Region / physiology Enhancer Elements, Genetic / genetics* Gene Expression Regulation, Developmental Green Fluorescent Proteins / genetics Homeodomain Proteins / genetics* Neurons / classification* Neurons / physiology* Reticular Formation / cytology Reticular Formation / embryology Reticular Formation / physiology Rhombencephalon / cytology Rhombencephalon / embryology* Rhombencephalon / physiology Transgenes / genetics Vagus Nerve / cytology Vagus Nerve / embryology Vagus Nerve / physiology Zebrafish Zebrafish Proteins / genetics*
IF 2.507
Times Cited 12
WOS Category ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY
Resource
Zebrafish Tg(isl1:GFP)rw0