論文 - 詳細
| RRC ID | 56061 |
|---|---|
| 著者 | Rawangkan A, Wongsirisin P, Namiki K, Iida K, Kobayashi Y, Shimizu Y, Fujiki H, Suganuma M. |
| タイトル | Green Tea Catechin Is an Alternative Immune Checkpoint Inhibitor that Inhibits PD-L1 Expression and Lung Tumor Growth. |
| ジャーナル | Molecules |
| Abstract |
The anticancer activity of immune checkpoint inhibitors is attracting attention in various clinical sites. Since green tea catechin has cancer-preventive activity in humans, whether green tea catechin supports the role of immune checkpoint inhibitors was studied. We here report that (-)-epigallocatechin gallate (EGCG) inhibited programmed cell death ligand 1 (PD-L1) expression in non⁻small-cell lung cancer cells, induced by both interferon (IFN)-γ and epidermal growth factor (EGF). The mRNA and protein levels of IFN-γ⁻induced PD-L1 were reduced 40⁻80% after pretreatment with EGCG and green tea extract (GTE) in A549 cells, via inhibition of JAK2/STAT1 signaling. Similarly, EGF-induced PD-L1 expression was reduced about 37⁻50% in EGCG-pretreated Lu99 cells through inhibition of EGF receptor/Akt signaling. Furthermore, 0.3% GTE in drinking water reduced the average number of tumors per mouse from 4.1 ± 0.5 to 2.6 ± 0.4 and the percentage of PD-L1 positive cells from 9.6% to 2.9%, a decrease of 70%, in lung tumors of A/J mice given a single intraperitoneal injection of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). In co-culture experiments using F10-OVA melanoma cells and tumor-specific CD3+ T cells, EGCG reduced PD-L1 mRNA expression about 30% in F10-OVA cells and restored interleukin-2 mRNA expression in tumor-specific CD3+ T cells. The results show that green tea catechin is an immune checkpoint inhibitor. |
| 巻・号 | 23(8) |
| 公開日 | 2018-8-18 |
| DOI | 10.3390/molecules23082071 |
| PII | molecules23082071 |
| PMID | 30126206 |
| PMC | PMC6222340 |
| MeSH | Animals Antineoplastic Agents, Phytogenic / chemistry Antineoplastic Agents, Phytogenic / pharmacology* B7-H1 Antigen / antagonists & inhibitors* Catechin / chemistry Catechin / pharmacology* Cell Line, Tumor Disease Models, Animal Gene Expression Humans Immunomodulation / drug effects* Lung Neoplasms / genetics Lung Neoplasms / immunology Lung Neoplasms / metabolism Lung Neoplasms / pathology Mice Phosphorylation Proto-Oncogene Proteins c-akt / metabolism STAT1 Transcription Factor / metabolism Signal Transduction / drug effects T-Lymphocyte Subsets / drug effects T-Lymphocyte Subsets / immunology T-Lymphocyte Subsets / metabolism Tea / chemistry* Xenograft Model Antitumor Assays |
| IF | 3.06 |
| 引用数 | 21 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Lu99(RCB1900) |