RRC ID 56099
著者 Matsushima Y, Terada K, Takata J, Karube Y, Kamei C, Sugimoto Y.
タイトル Effects of fluvoxamine on nerve growth factor-induced neurite outgrowth inhibition by dexamethasone in PC12 cells.
ジャーナル Biosci Biotechnol Biochem
Abstract In the present study, we examined the effects of fluvoxamine on nerve growth factor (NGF)-induced neurite outgrowth inhibition by dexamethasone (DEX) in PC12 cells. Fluvoxamine increased NGF-induced neurite outgrowth. Compared with co-treatment with NGF and fluvoxamine, p-Akt levels were higher than the values without fluvoxamine. The phosphorylated extracellular regulated kinase 1/2 levels were slightly increased by co-treatment with NGF and fluvoxamine. Fluvoxamine concentration-dependently improved NGF-induced neurite outgrowth inhibition by DEX. Fluvoxamine also improved the decrease in the NGF-induced p-Akt level caused by DEX. Interestingly, the sigma-1 receptor antagonist NE-100 blocked the improvement effects of fluvoxamine on NGF-induced neurite outgrowth inhibition by DEX. The selective sigma-1 receptor agonist PRE-084 also improved NGF-induced neurite outgrowth inhibition by DEX, which is blocked by NE-100. These results indicate that the improvement effects of fluvoxamine on NGF-induced neurite outgrowth inhibition by DEX may be attributable to the phosphorylation of Akt and the sigma-1 receptor.
巻・号 83(4)
ページ 659-665
公開日 2019-4-1
DOI 10.1080/09168451.2018.1553607
PMID 30543144
MeSH Animals Anisoles / pharmacology Anti-Anxiety Agents / pharmacology* Cell Differentiation / drug effects Dexamethasone / antagonists & inhibitors Dexamethasone / pharmacology Fluvoxamine / pharmacology* Gene Expression Regulation / drug effects* Glucocorticoids / antagonists & inhibitors Glucocorticoids / pharmacology Mitogen-Activated Protein Kinase 1 / genetics Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / genetics Mitogen-Activated Protein Kinase 3 / metabolism Morpholines / pharmacology Nerve Growth Factor / pharmacology Neuronal Outgrowth / drug effects* Neuronal Outgrowth / genetics Neurons / cytology Neurons / drug effects* Neurons / metabolism PC12 Cells Phosphorylation / drug effects Propylamines / pharmacology Proto-Oncogene Proteins c-akt / agonists Proto-Oncogene Proteins c-akt / antagonists & inhibitors Proto-Oncogene Proteins c-akt / genetics* Proto-Oncogene Proteins c-akt / metabolism Rats Receptors, sigma / agonists Receptors, sigma / antagonists & inhibitors Receptors, sigma / genetics Receptors, sigma / metabolism Signal Transduction
IF 1.297
リソース情報
ヒト・動物細胞 PC-12(RCB0009)