論文 - 詳細
| RRC ID | 56113 |
|---|---|
| 著者 | Tanaka M, Kishimoto Y, Sasaki M, Sato A, Kamiya T, Kondo K, Iida K. |
| タイトル | Terminalia bellirica (Gaertn.) Roxb. Extract and Gallic Acid Attenuate LPS-Induced Inflammation and Oxidative Stress via MAPK/NF-κB and Akt/AMPK/Nrf2 Pathways. |
| ジャーナル | Oxid Med Cell Longev |
| Abstract |
Excessive oxidative stress plays a critical role in the progression of various diseases. Recently, we showed that Terminalia bellirica (Gaertn.) Roxb. extract (TBE) inhibits inflammatory response and reactive oxygen species (ROS) production in THP-1 macrophages. However, molecular mechanisms underlying anti-inflammatory and antioxidant activities of TBE and its major polyphenolic compounds gallic acid (GA) and ellagic acid (EA) remain unclear. We found that TBE and GA attenuated LPS-induced inflammatory mediator expression, ROS production, and activation of mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) in RAW 264 macrophages. Furthermore, TBE and GA increased antioxidant enzyme expression along with upstream mediators nuclear factor erythroid-2-related factor 2 (Nrf2), Akt, and AMP-activated protein kinase (AMPK). Importantly, knockdown of Nrf2 by siRNA and specific inhibition of Akt and AMPK significantly reduced antioxidant enzyme expression induced by TBE and GA. Finally, in vivo effects on histopathology and gene expression were assessed in tissues collected after intraperitoneal injection of LPS with or without TBE treatment. TBE enhanced antioxidant enzyme expression and improved acute kidney injury in LPS-shock model mice. In conclusion, TBE and GA exert protective effects against inflammation and oxidative stress by suppressing MAPK/NF-κB pathway and by activating Akt/AMPK/Nrf2 pathway. These results suggest that TBE and GA might be effective for the treatment of inflammation-related diseases. |
| 巻・号 | 2018 |
| ページ | 9364364 |
| 公開日 | 2018-11-8 |
| DOI | 10.1155/2018/9364364 |
| PMID | 30533177 |
| PMC | PMC6250009 |
| MeSH | Adenylate Kinase / drug effects Adenylate Kinase / metabolism Animals Anti-Inflammatory Agents / pharmacology* Gallic Acid / pharmacology* Inflammation / chemically induced Inflammation / metabolism Lipopolysaccharides / toxicity MAP Kinase Signaling System / drug effects MAP Kinase Signaling System / physiology Male Mice Mice, Inbred ICR NF-E2-Related Factor 2 / drug effects NF-E2-Related Factor 2 / metabolism NF-kappa B / drug effects NF-kappa B / metabolism Oxidative Stress / drug effects* Plant Extracts / pharmacology* Proto-Oncogene Proteins c-akt / drug effects Proto-Oncogene Proteins c-akt / metabolism RAW 264.7 Cells Signal Transduction / drug effects* Terminalia |
| IF | 4.868 |
| 引用数 | 15 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |