Reference - Detail
| RRC ID | 56137 |
|---|---|
| Author | Ishibashi O, Akagi I, Ogawa Y, Inui T. |
| Title | MiR-141-3p is upregulated in esophageal squamous cell carcinoma and targets pleckstrin homology domain leucine-rich repeat protein phosphatase-2, a negative regulator of the PI3K/AKT pathway. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
The phosphatidylinositol-3-kinase (PI3K)/AKT pathway is frequently activated in various human cancers and plays essential roles in their development and progression. Accumulating evidence suggests that dysregulated expression of microRNAs (miRNAs) is closely associated with cancer progression and metastasis. Here, we focused on miRNAs that could regulate genes related to the PI3K/AKT pathway in esophageal squamous cell carcinoma (ESCC). To identify upregulated miRNAs and their possible target genes in ESCC, we performed microarray-based integrative analyses of miRNA and mRNA expression levels in three human ESCC cell lines and a normal esophageal epithelial cell line. The miRNA microarray analysis revealed that miR-31-5p, miR-141-3p, miR-200b-3p, miR-200c-3p, and miR-205-5p were expressed at higher levels in the ESCC cell lines than the normal esophageal epithelial cell line. Bioinformatical analyses of mRNA microarray data identified several AKT/PI3K pathway-related genes as candidate targets of these miRNAs, which include tumor suppressors such as DNA-damage-inducible transcript 4 and pleckstrin homology domain leucine-rich repeat protein phosphatase-2 (PHLPP2). To validate the targets of relevant miRNAs experimentally, synthetic mimics of the miRNAs were transfected into the esophageal epithelial cell line. Here, we report that miR-141-3p suppress the expression of PHLPP2, a negative regulators of the AKT/PI3K pathway, as a target in ESCC. |
| Volume | 501(2) |
| Pages | 507-513 |
| Published | 2018-6-22 |
| DOI | 10.1016/j.bbrc.2018.05.025 |
| PII | S0006-291X(18)31074-X |
| PMID | 29738771 |
| MeSH | 3' Untranslated Regions Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / metabolism Cell Line, Tumor Esophageal Neoplasms / genetics* Esophageal Neoplasms / metabolism Esophageal Squamous Cell Carcinoma Gene Expression Regulation, Neoplastic* Humans MicroRNAs / genetics* Phosphatidylinositol 3-Kinases / genetics* Phosphatidylinositol 3-Kinases / metabolism Phosphoprotein Phosphatases / genetics* Phosphoprotein Phosphatases / metabolism Proto-Oncogene Proteins c-akt / genetics* Proto-Oncogene Proteins c-akt / metabolism Signal Transduction Up-Regulation |
| IF | 2.705 |
| Times Cited | 12 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | TE-1(RCB1894) TE-5(RCB1949) TE-8(RCB2098) |