Reference - Detail
| RRC ID | 56172 |
|---|---|
| Author | Hsu TY, Butler VJ, Kao AW. |
| Title | The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response. |
| Journal | Methods Mol Biol |
| Abstract |
The nematode Caenorhabditis elegans (C. elegans) has proven to be a powerful model organism for the study of many biological processes, with major implications for human health and disease. As progranulin is a pleiotropic, secreted protein with both cell autonomous and non-autonomous roles, a multicellular organism such as C. elegans is ideal for the investigation of its normal function and pathological effects. The C. elegans genome contains a progranulin-like gene known as pgrn-1. The nematode pgrn-1 encodes a protein with three cysteine-rich granulin domains, compared to the seven and a half granulins in the human protein. We have shown that C. elegans mutants lacking pgrn-1 appear grossly normal, but exhibit accelerated apoptotic cell engulfment as well as a stress resistance phenotype (Kao et al., Proc Natl Acad Sci U S A 108:4441-4446, 2011; Judy et al., PLoS Genet 9:e1003714, 2013). In addition, the roles of individual granulins can also be dissected in C. elegans (Salazar et al., J Neurosci 35:9315-9328, 2015). Here, we describe methods for studying apoptosis and stress response in C. elegans. |
| Volume | 1806 |
| Pages | 193-206 |
| Published | 2018-1-1 |
| DOI | 10.1007/978-1-4939-8559-3_14 |
| PMID | 29956278 |
| MeSH | Animals Apoptosis* Biological Assay Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology Caenorhabditis elegans / physiology* Embryonic Development Humans Kinetics Larva / cytology Larva / metabolism Osmotic Pressure Progranulins / chemistry Progranulins / metabolism* Stress, Physiological* Time-Lapse Imaging |
| Times Cited | 0 |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
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| C.elegans | NA |