RRC ID 56222
Author Shen P, Kershaw JC, Yue Y, Wang O, Kim KH, McClements DJ, Park Y.
Title Effects of conjugated linoleic acid (CLA) on fat accumulation, activity, and proteomics analysis in Caenorhabditis elegans.
Journal Food Chem
Abstract Conjugated linoleic acid (CLA) has been reported to reduce fat storage in cell culture and animal models. In the current study, the effects of CLA on the fat accumulation, activities, and proteomics were investigated using Caenorhabditis elegans. 100 µM CLA-TG nanoemulsion significantly reduced fat accumulation by 29% compared to linoleic acid (LA)-TG treatment via sir-2.1 (the ortholog of Sirtuin 1), without altering the worm size, growth rate, and pumping rate of C. elegans. CLA significantly increased moving speed and amplitude (the average centroid displacement over the entire track) of wild type worms compared to the LA group and these effects were dependent on aak-2 (AMPKα ortholog) and sir-2.1. Proteomics analysis showed CLA treatment influences various proteins associated in reproduction and development, translation, metabolic processes, and catabolism and proteolysis, in C. elegans. We have also confirmed the proteomics data that CLA reduced the fat accumulation via abs-1 (ATP Synthase B homolog). However, there were no significant effects of CLA on brood size, progeny numbers, and hatchability compared to LA treatment.
Volume 249
Pages 193-201
Published 2018-5-30
DOI 10.1016/j.foodchem.2018.01.017
PII S0308-8146(18)30017-7
PMID 29407924
MeSH Adipocytes / chemistry Adipocytes / metabolism Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism Fats / metabolism* Linoleic Acid / metabolism Linoleic Acids, Conjugated / metabolism* Models, Animal Proteomics
IF 5.399
Times Cited 9
C.elegans tm498