論文 - 詳細
| RRC ID | 56229 |
|---|---|
| 著者 | Uebel CJ, Anderson DC, Mandarino LM, Manage KI, Aynaszyan S, Phillips CM. |
| タイトル | Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci. |
| ジャーナル | PLoS Genet |
| Abstract |
In C. elegans, efficient RNA silencing requires small RNA amplification mediated by RNA-dependent RNA polymerases (RdRPs). RRF-1, an RdRP, and other Mutator complex proteins localize to Mutator foci, which are perinuclear germline foci that associate with nuclear pores and P granules to facilitate small RNA amplification. The Mutator complex protein MUT-16 is critical for Mutator foci assembly. By analyzing small deletions of MUT-16, we identify specific regions of the protein that recruit other Mutator complex components and demonstrate that it acts as a scaffolding protein. We further determine that the C-terminal region of MUT-16, a portion of which contains predicted intrinsic disorder, is necessary and sufficient to promote Mutator foci formation. Finally, we establish that MUT-16 foci have many properties consistent with a phase-separated condensate and propose that Mutator foci form through liquid-liquid phase separation of MUT-16. P granules, which contain additional RNA silencing proteins, have previously been shown to have liquid-like properties. Thus, RNA silencing in C. elegans germ cells may rely on multiple phase-separated compartments through which sorting, processing, and silencing of mRNAs occurs. |
| 巻・号 | 14(7) |
| ページ | e1007542 |
| 公開日 | 2018-7-1 |
| DOI | 10.1371/journal.pgen.1007542 |
| PII | PGENETICS-D-18-00011 |
| PMID | 30036386 |
| PMC | PMC6072111 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / genetics Germ-Line Mutation Intrinsically Disordered Proteins / genetics Intrinsically Disordered Proteins / metabolism* RNA Interference / physiology* RNA-Dependent RNA Polymerase / genetics RNA-Dependent RNA Polymerase / metabolism* |
| IF | 5.224 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 線虫 | tm4844 tm5004 tm2252 tm1358 tm1301 |