RRC ID 56229
著者 Uebel CJ, Anderson DC, Mandarino LM, Manage KI, Aynaszyan S, Phillips CM.
タイトル Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci.
ジャーナル PLoS Genet
Abstract In C. elegans, efficient RNA silencing requires small RNA amplification mediated by RNA-dependent RNA polymerases (RdRPs). RRF-1, an RdRP, and other Mutator complex proteins localize to Mutator foci, which are perinuclear germline foci that associate with nuclear pores and P granules to facilitate small RNA amplification. The Mutator complex protein MUT-16 is critical for Mutator foci assembly. By analyzing small deletions of MUT-16, we identify specific regions of the protein that recruit other Mutator complex components and demonstrate that it acts as a scaffolding protein. We further determine that the C-terminal region of MUT-16, a portion of which contains predicted intrinsic disorder, is necessary and sufficient to promote Mutator foci formation. Finally, we establish that MUT-16 foci have many properties consistent with a phase-separated condensate and propose that Mutator foci form through liquid-liquid phase separation of MUT-16. P granules, which contain additional RNA silencing proteins, have previously been shown to have liquid-like properties. Thus, RNA silencing in C. elegans germ cells may rely on multiple phase-separated compartments through which sorting, processing, and silencing of mRNAs occurs.
巻・号 14(7)
ページ e1007542
公開日 2018-7-1
DOI 10.1371/journal.pgen.1007542
PII PGENETICS-D-18-00011
PMID 30036386
PMC PMC6072111
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / genetics Germ-Line Mutation Intrinsically Disordered Proteins / genetics Intrinsically Disordered Proteins / metabolism* RNA Interference / physiology* RNA-Dependent RNA Polymerase / genetics RNA-Dependent RNA Polymerase / metabolism*
IF 5.224
引用数 9
リソース情報
線虫 tm4844 tm5004 tm2252 tm1358 tm1301