RRC ID 5640
Author Park JS, Kim YS, Yoo MA.
Title The role of p38b MAPK in age-related modulation of intestinal stem cell proliferation and differentiation in Drosophila.
Journal Aging (Albany NY)
Abstract It is important to understand how age-related changes in intestinal stem cells (ISCs) may contribute to age-associated intestinal diseases, including cancer. Drosophila midgut is an excellent model system for the study of ISC proliferation and differentiation. Recently, age-related changes in the Drosophila midgut have been shown to include an increase in ISC proliferation and accumulation of mis-differentiated ISC daughter cells. Here, we show that the p38b MAPK pathway contributes to the age-related changes in ISC and progenitor cells in Drosophila. D-p38b MAPK is required for an age-related increase of ISC proliferation. In addition, this pathway is involved in age and oxidative stress-associated mis-differentiation of enterocytes and upregulation of Delta, a Notch receptor ligand. Furthermore, we also show that D-p38b acts downstream of PVF2/PVR signaling in these age-related changes. Taken together, our findings suggest that p38 MAPK plays a crucial role in the balance between ISC proliferation and proper differentiation in the adult Drosophila midgut.
Volume 1(7)
Pages 637-51
Published 2009-5-21
DOI 10.18632/aging.100054
PMID 20157545
PMC PMC2806044
MeSH Adult Stem Cells / cytology* Adult Stem Cells / metabolism Aging / physiology* Animals Animals, Genetically Modified / physiology Cell Differentiation / drug effects Cell Differentiation / physiology* Cell Proliferation* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / enzymology* Enterocytes / cytology Enterocytes / metabolism Enteroendocrine Cells / cytology Enteroendocrine Cells / metabolism Gene Expression / genetics Intestinal Mucosa / metabolism Intestines / cytology* Intracellular Signaling Peptides and Proteins Membrane Proteins / genetics Membrane Proteins / metabolism Mitogen-Activated Protein Kinase 11 / genetics Mitogen-Activated Protein Kinase 11 / metabolism* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Nuclear Proteins / genetics Nuclear Proteins / metabolism Oxidative Stress / physiology Paraquat / pharmacology Receptor Protein-Tyrosine Kinases / genetics Receptor Protein-Tyrosine Kinases / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism Signal Transduction / physiology Stem Cells / cytology Stem Cells / metabolism Transcription Factors / genetics Transcription Factors / metabolism
IF 5.515
Times Cited 48
Drosophila esg-GAL4