RRC ID 565
著者 Ohsaki A, Iiyama K, Miyagawa Y, Kawaguchi Y, Koga K, Kusakabe T.
タイトル Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4.
ジャーナル Mol Biol Rep
Abstract Nonhomologous end-joining (NHEJ) is one of the repair pathways for double-strand breaks (DSBs) in eukaryotic cells. By using linearized plasmid substrates, we have detected intramolecular NHEJ activity in a cell-free extract from the cultured silkworm cell line BmN4. The efficiency of NHEJ differed according to the structure of DNA ends; approximately 1% of input DNA was repaired when the substrate had cohesive ends. The reaction required the hydrolysis of nucleotide triphosphate; interestingly, all of four rNTPs or four dNTPs could support the reaction. A substrate with non-complementary DNA ends was mainly repaired by the DNA polymerase-mediated pathway. These results indicate that the present cell-free system will be useful to analyze the molecular mechanisms of DSB repair and NHEJ in insect cells.
巻・号 32(1)
ページ 25-34
公開日 2005-3-1
DOI 10.1007/s11033-004-2474-y
PMID 15865207
MeSH Animals Bombyx / chemistry Bombyx / cytology Bombyx / genetics* Cations, Divalent / chemistry Cell Line Cell-Free System DNA Damage* DNA Repair* Metals / chemistry Plasmids / chemistry Plasmids / genetics Recombination, Genetic
IF 1.402
引用数 2
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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