論文 - 詳細
| RRC ID | 56625 |
|---|---|
| 著者 | Momozane T, Kawamura T, Itoh Y, Sanosaka M, Sasaki T, Kanzaki R, Ose N, Funaki S, Shintani Y, Minami M, Okumura M, Takemori H. |
| タイトル | Carnosol suppresses interleukin-6 production in mouse lungs injured by ischemia-reperfusion operation and in RAW264.7 macrophages treated with lipopolysaccharide. |
| ジャーナル | Biochem Cell Biol |
| Abstract |
Carnosol is a naturally occurring herbal compound, known for its antioxidative properties. We previously found that carnosol protected mouse lungs from ischemia-reperfusion injury in ex vivo cultures. To elucidate the molecular mechanisms underpinning carnosol-mediated lung protection, we analyzed modes of interleukin-6 (IL-6) gene expression, which is associated with lung ischemia-reperfusion injury. Microarray analysis of mouse lungs suggested that IL-6 mRNA levels were elevated in the mouse lungs subjected to clamp-reperfusion, which was associated with elevated levels of other inflammatory modulators, such as activating transcription factor 3 (ATF3). Carnosol pretreatment lowered the IL-6 protein levels in mouse lung homogenates prepared after the clamp-reperfusion. On the other hand, the ATF3 gene expression was negatively correlated with that of IL-6 in RAW264.7 cells. IL-6 mRNA levels and gene promoter activities were suppressed by carnosol in RAW264.7 cells, but rescued by ATF3 knockdown. When RAW264.7 cells were subjected to hypoxia-reoxygenation, carnosol treatment lowered oxygen consumption after reoxygenation, which was coupled with a correlation with a transient production of mitochondrial reactive oxygen species and following ATF3 gene expression. These results suggest that carnosol treatment could be a new strategy for protecting lungs from ischemia-reperfusion injury by modulating the ATF3-IL-6 axis. |
| 巻・号 | 96(6) |
| ページ | 769-776 |
| 公開日 | 2018-12-1 |
| DOI | 10.1139/bcb-2017-0339 |
| PMID | 29958095 |
| MeSH | Abietanes / pharmacology* Animals Cells, Cultured Dose-Response Relationship, Drug Interleukin-6 / antagonists & inhibitors* Interleukin-6 / biosynthesis Interleukin-6 / genetics Lipopolysaccharides / pharmacology* Lung / metabolism* Lung / pathology Macrophages / drug effects* Macrophages / metabolism Mice Mice, Inbred C57BL RAW 264.7 Cells RNA, Messenger / antagonists & inhibitors RNA, Messenger / biosynthesis RNA, Messenger / genetics Reactive Oxygen Species / analysis Reactive Oxygen Species / metabolism Reperfusion Injury / metabolism* Reperfusion Injury / pathology |
| IF | 2.014 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |