RRC ID 56859
著者 Dress RJ, Dutertre CA, Giladi A, Schlitzer A, Low I, Shadan NB, Tay A, Lum J, Kairi MFBM, Hwang YY, Becht E, Cheng Y, Chevrier M, Larbi A, Newell EW, Amit I, Chen J, Ginhoux F.
タイトル Plasmacytoid dendritic cells develop from Ly6D+ lymphoid progenitors distinct from the myeloid lineage.
ジャーナル Nat Immunol
Abstract Dendritic cells (DC) are currently classified as conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Through a combination of single-cell transcriptomic analysis, mass cytometry, in vivo fate mapping and in vitro clonal assays, here we show that, at the single-cell level, the priming of mouse hematopoietic progenitor cells toward the pDC lineage occurs at the common lymphoid progenitor stage, indicative of early divergence of the pDC and cDC lineages. We found the transcriptional signature of a pDC precursor stage, defined here, in the IL-7Rα+ common lymphoid progenitor population and identified Ly6D, IL-7Rα, CD81 and CD2 as key markers of pDC differentiation, which distinguish pDC precursors from cDC precursors. In conclusion, pDCs developed in the bone marrow from a Ly6DhiCD2hi lymphoid progenitor cell and differentiated independently of the myeloid cDC lineage.
巻・号 20(7)
ページ 852-864
公開日 2019-7-1
DOI 10.1038/s41590-019-0420-3
PII 10.1038/s41590-019-0420-3
PMID 31213723
MeSH Animals Antigens, Ly / metabolism* B-Lymphocytes / immunology B-Lymphocytes / metabolism Biomarkers Bone Marrow Cells / cytology Bone Marrow Cells / metabolism Cell Differentiation / genetics Cell Differentiation / immunology Dendritic Cells / cytology* Dendritic Cells / metabolism* Flow Cytometry GPI-Linked Proteins / metabolism Gene Expression Gene Expression Profiling Lymphoid Progenitor Cells / cytology* Lymphoid Progenitor Cells / metabolism* Mice Myeloid Progenitor Cells / cytology* Myeloid Progenitor Cells / metabolism* Transcriptome
IF 23.53
引用数 26
リソース情報
実験動物マウス RBRC02705