論文 - 詳細
| RRC ID | 56901 |
|---|---|
| 著者 | Yamamoto K, Yamada N. |
| タイトル | Identification of a diazinon-metabolizing glutathione S-transferase in the silkworm, Bombyx mori. |
| ジャーナル | Sci Rep |
| Abstract |
The glutathione S-transferase superfamily play key roles in the metabolism of numerous xenobiotics. We report herein the identification and characterization of a novel glutathione S-transferase in the silkworm, Bombyx mori. The enzyme (bmGSTu2) conjugates glutathione to 1-chloro-2,4-dinitrobenzene, as well as metabolizing diazinon, one of the organophosphate insecticides. Quantitative reverse transcription-polymerase chain reaction analysis of transcripts demonstrated that bmGSTu2 expression was induced 1.7-fold in a resistant strain of B. mori. Mutagenesis of putative amino acid residues in the glutathione-binding site revealed that Ile54, Glu66, Ser67, and Asn68 are crucial for enzymatic function. These results provide insights into the catalysis of glutathione conjugation in silkworm by bmGSTu2 and into the detoxification of organophosphate insecticides. |
| 巻・号 | 6 |
| ページ | 30073 |
| 公開日 | 2016-7-21 |
| DOI | 10.1038/srep30073 |
| PII | srep30073 |
| PMID | 27440377 |
| PMC | PMC4954967 |
| MeSH | Animals Binding Sites Biotransformation Bombyx / enzymology* DNA Mutational Analysis Diazinon / metabolism* Dinitrochlorobenzene / metabolism Gene Expression Profiling Glutathione / metabolism Glutathione Transferase / genetics Glutathione Transferase / metabolism* Insecticides / metabolism* |
| IF | 4.011 |
| 引用数 | 15 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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