| RRC ID |
57064
|
| 著者 |
Yamaya-Ito H, Shimoda Y, Hakoyama T, Sato S, Kaneko T, Hossain MS, Shibata S, Kawaguchi M, Hayashi M, Kouchi H, Umehara Y.
|
| タイトル |
Loss-of-function of ASPARTIC PEPTIDASE NODULE-INDUCED 1 (APN1) in Lotus japonicus restricts efficient nitrogen-fixing symbiosis with specific Mesorhizobium loti strains.
|
| ジャーナル |
Plant J
|
| Abstract |
The nitrogen-fixing symbiosis of legumes and Rhizobium bacteria is established by complex interactions between the two symbiotic partners. Legume Fix- mutants form apparently normal nodules with endosymbiotic rhizobia but fail to induce rhizobial nitrogen fixation. These mutants are useful for identifying the legume genes involved in the interactions essential for symbiotic nitrogen fixation. We describe here a Fix- mutant of Lotus japonicus, apn1, which showed a very specific symbiotic phenotype. It formed ineffective nodules when inoculated with the Mesorhizobium loti strain TONO. In these nodules, infected cells disintegrated and successively became necrotic, indicating premature senescence typical of Fix- mutants. However, it formed effective nodules when inoculated with the M. loti strain MAFF303099. Among nine different M. loti strains tested, four formed ineffective nodules and five formed effective nodules on apn1 roots. The identified causal gene, ASPARTIC PEPTIDASE NODULE-INDUCED 1 (LjAPN1), encodes a nepenthesin-type aspartic peptidase. The well characterized Arabidopsis aspartic peptidase CDR1 could complement the strain-specific Fix- phenotype of apn1. LjAPN1 is a typical late nodulin; its gene expression was exclusively induced during nodule development. LjAPN1 was most abundantly expressed in the infected cells in the nodules. Our findings indicate that LjAPN1 is required for the development and persistence of functional (nitrogen-fixing) symbiosis in a rhizobial strain-dependent manner, and thus determines compatibility between M. loti and L. japonicus at the level of nitrogen fixation.
|
| 巻・号 |
93(1)
|
| ページ |
5-16
|
| 公開日 |
2018-1-1
|
| DOI |
10.1111/tpj.13759
|
| PMID |
29086445
|
| MeSH |
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
Aspartic Acid Endopeptidases / genetics
Aspartic Acid Endopeptidases / metabolism
Aspartic Acid Proteases / genetics
Aspartic Acid Proteases / metabolism*
Loss of Function Mutation
Lotus / enzymology*
Lotus / genetics
Lotus / microbiology
Lotus / physiology
Mesorhizobium / physiology*
Nitrogen / metabolism*
Nitrogen Fixation
Phenotype
Plant Proteins / genetics
Plant Proteins / metabolism
Rhizobium / physiology*
Root Nodules, Plant / enzymology
Root Nodules, Plant / genetics
Root Nodules, Plant / microbiology
Root Nodules, Plant / physiology
Species Specificity
Symbiosis*
|
| IF |
6.141
|
| 引用数 |
20
|
| リソース情報 |
| ミヤコグサ・ダイズ |
ミヤコグサDNA |
