論文 - 詳細
| RRC ID | 57089 |
|---|---|
| 著者 | Wolfstetter G, Dahlitz I, Pfeifer K, Töpfer U, Alt JA, Pfeifer DC, Lakes-Harlan R, Baumgartner S, Palmer RH, Holz A. |
| タイトル | Characterization of Drosophila Nidogen/entactin reveals roles in basement membrane stability, barrier function and nervous system patterning. |
| ジャーナル | Development |
| Abstract |
Basement membranes (BMs) are specialized layers of extracellular matrix (ECM) mainly composed of Laminin, type IV Collagen, Perlecan and Nidogen/entactin (NDG). Recent in vivo studies challenged the initially proposed role of NDG as a major ECM linker molecule by revealing dispensability for viability and BM formation. Here, we report the characterization of the single Ndg gene in Drosophila. Embryonic Ndg expression was primarily observed in mesodermal tissues and the chordotonal organs, whereas NDG protein localized to all BMs. Although loss of Laminin strongly affected BM localization of NDG, Ndg-null mutants exhibited no overt changes in the distribution of BM components. Although Drosophila Ndg mutants were viable, loss of NDG led to ultrastructural BM defects that compromised barrier function and stability in vivo Moreover, loss of NDG impaired larval crawling behavior and reduced responses to vibrational stimuli. Further morphological analysis revealed accompanying defects in the larval peripheral nervous system, especially in the chordotonal organs and the neuromuscular junction (NMJ). Taken together, our analysis suggests that NDG is not essential for BM assembly but mediates BM stability and ECM-dependent neural plasticity during Drosophila development. |
| 巻・号 | 146(2) |
| 公開日 | 2019-1-16 |
| DOI | 10.1242/dev.168948 |
| PII | dev.168948 |
| PMID | 30567930 |
| MeSH | Animals Basement Membrane / metabolism* Basement Membrane / ultrastructure Behavior, Animal Biomechanical Phenomena Body Patterning* Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Embryo, Nonmammalian / metabolism Embryonic Development / genetics Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism* Gene Deletion Gene Expression Regulation, Developmental Laminin / metabolism Larva / genetics Nervous System / embryology* Nervous System / metabolism* Neuromuscular Junction / pathology Peripheral Nervous System / embryology Peripheral Nervous System / pathology Permeability Phenotype RNA, Messenger / genetics RNA, Messenger / metabolism Vibration |
| IF | 5.763 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | HMC02910 |