Reference - Detail
RRC ID | 5710 |
---|---|
Author | Broitman-Maduro G, Owraghi M, Hung WW, Kuntz S, Sternberg PW, Maduro MF. |
Title | The NK-2 class homeodomain factor CEH-51 and the T-box factor TBX-35 have overlapping function in C. elegans mesoderm development. |
Journal | Development |
Abstract |
The C. elegans MS blastomere, born at the 7-cell stage of embryogenesis, generates primarily mesodermal cell types, including pharynx cells, body muscles and coelomocytes. A presumptive null mutation in the T-box factor gene tbx-35, a target of the MED-1 and MED-2 divergent GATA factors, was previously found to result in a profound decrease in the production of MS-derived tissues, although the tbx-35(-) embryonic arrest phenotype was variable. We report here that the NK-2 class homeobox gene ceh-51 is a direct target of TBX-35 and at least one other factor, and that CEH-51 and TBX-35 share functions. Embryos homozygous for a ceh-51 null mutation arrest as larvae with pharynx and muscle defects, although these tissues appear to be specified correctly. Loss of tbx-35 and ceh-51 together results in a synergistic phenotype resembling loss of med-1 and med-2. Overexpression of ceh-51 causes embryonic arrest and generation of ectopic body muscle and coelomocytes. Our data show that TBX-35 and CEH-51 have overlapping function in MS lineage development. As T-box regulators and NK-2 homeodomain factors are both important for heart development in Drosophila and vertebrates, our results suggest that these regulators function in a similar manner in C. elegans to specify a major precursor of mesoderm. |
Volume | 136(16) |
Pages | 2735-46 |
Published | 2009-8-1 |
DOI | 10.1242/dev.038307 |
PII | dev.038307 |
PMID | 19605496 |
PMC | PMC2730403 |
MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Blastomeres / physiology Caenorhabditis elegans / anatomy & histology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Lineage Gene Expression Regulation, Developmental Gene Knockout Techniques Gene Regulatory Networks / physiology Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Mesoderm / physiology* Molecular Sequence Data Phenotype RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Alignment T-Box Domain Proteins / genetics T-Box Domain Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* |
IF | 5.611 |
Times Cited | 33 |
WOS Category | DEVELOPMENTAL BIOLOGY |
Resource | |
C.elegans | tm2123 |