RRC ID 57133
著者 Lőrincz P, Kenéz LA, Tóth S, Kiss V, Varga Á, Csizmadia T, Simon-Vecsei Z, Juhász G.
タイトル Vps8 overexpression inhibits HOPS-dependent trafficking routes by outcompeting Vps41/Lt.
ジャーナル Elife
Abstract Two related multisubunit tethering complexes promote endolysosomal trafficking in all eukaryotes: Rab5-binding CORVET that was suggested to transform into Rab7-binding HOPS. We have previously identified miniCORVET, containing Drosophila Vps8 and three shared core proteins, which are required for endosome maturation upstream of HOPS in highly endocytic cells (Lőrincz et al., 2016a). Here, we show that Vps8 overexpression inhibits HOPS-dependent trafficking routes including late endosome maturation, autophagosome-lysosome fusion, crinophagy and lysosome-related organelle formation. Mechanistically, Vps8 overexpression abolishes the late endosomal localization of HOPS-specific Vps41/Lt and prevents HOPS assembly. Proper ratio of Vps8 to Vps41 is thus critical because Vps8 negatively regulates HOPS by outcompeting Vps41. Endosomal recruitment of miniCORVET- or HOPS-specific subunits requires proper complex assembly, and Vps8/miniCORVET is dispensable for autophagy, crinophagy and lysosomal biogenesis. These data together indicate the recruitment of these complexes to target membranes independent of each other in Drosophila, rather than their transformation during vesicle maturation.
巻・号 8
公開日 2019-6-13
DOI 10.7554/eLife.45631
PII 45631
PMID 31194677
PMC PMC6592680
MeSH Animals Drosophila Proteins / metabolism* Drosophila melanogaster* Endosomes / metabolism* Gene Expression* Vesicular Transport Proteins / metabolism*
IF 7.551
引用数 5
リソース情報
ショウジョウバエ 10144R-1 7891R-2 DGRC#141824 DGRC#141970