RRC ID 57208
Author Del Signore SJ, Cilla R, Hatini V.
Title The WAVE Regulatory Complex and Branched F-Actin Counterbalance Contractile Force to Control Cell Shape and Packing in the Drosophila Eye.
Journal Dev Cell
Abstract Contractile forces eliminate cell contacts in many morphogenetic processes. However, mechanisms that balance contractile forces to promote subtler remodeling remain unknown. To address this gap, we investigated remodeling of Drosophila eye lattice cells (LCs), which preserve cell contacts as they narrow to form the edges of a multicellular hexagonal lattice. We found that during narrowing, LC-LC contacts dynamically constrict and expand. Similar to other systems, actomyosin-based contractile forces promote pulses of constriction. Conversely, we found that WAVE-dependent branched F-actin accumulates at LC-LC contacts during expansion and functions to expand the cell apical area, promote shape changes, and prevent elimination of LC-LC contacts. Finally, we found that small Rho GTPases regulate the balance of contractile and protrusive dynamics. These data suggest a mechanism by which WAVE regulatory complex-based F-actin dynamics antagonize contractile forces to regulate cell shape and tissue topology during remodeling and thus contribute to the robustness and precision of the process.
Volume 44(4)
Pages 471-483.e4
Published 2018-2-26
DOI 10.1016/j.devcel.2017.12.025
PII S1534-5807(17)31042-0
PMID 29396116
PMC PMC5856465
MeSH Actin Cytoskeleton / metabolism* Actins / metabolism* Actomyosin / metabolism Animals Cell Shape Cells, Cultured Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism Epithelium / growth & development Epithelium / metabolism Eye / cytology* Eye / metabolism Female Male Morphogenesis / physiology* Muscle Contraction / physiology Wiskott-Aldrich Syndrome Protein Family / metabolism* rho GTP-Binding Proteins / metabolism
IF 9.19
Times Cited 7