RRC ID 57212
Author Wang Q, Wang Y, Yu F.
Title Yif1 associates with Yip1 on Golgi and regulates dendrite pruning in sensory neurons during Drosophila metamorphosis.
Journal Development
Abstract Pruning that selectively removes unnecessary neurites without causing neuronal death is essential for sculpting the mature nervous system during development. In Drosophila, ddaC sensory neurons specifically prune their larval dendrites with intact axons during metamorphosis. However, the important role of endoplasmic reticulum (ER)-to-Golgi transport in dendrite pruning remains unknown. Here, in a clonal screen, we have identified Yif1, an uncharacterized Drosophila homolog of Yif1p that is known to be a regulator of ER-to-Golgi transport in yeast. We show that Yif1 is required for dendrite pruning of ddaC neurons but not for apoptosis of ddaF neurons. We further identify that the Yif1-binding partner Yip1 is also crucial for dendrite pruning. Yif1 forms a protein complex with Yip1 in S2 cells and ddaC neurons. Yip1 and Yif1 colocalize on ER/Golgi and are required for the integrity of Golgi apparatus and outposts. Moreover, we show that two GTPases, Rab1 and Sar1, which are known to regulate ER-to-Golgi transport, are essential for dendrite pruning of ddaC neurons. Finally, our data reveal that ER-to-Golgi transport promotes endocytosis and downregulation of the cell-adhesion molecule Neuroglian and thereby dendrite pruning.
Volume 145(12)
Published 2018-6-13
DOI 10.1242/dev.164475
PII dev.164475
PMID 29769219
MeSH Animals Cell Adhesion Molecules, Neuronal / biosynthesis Dendrites / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism Endocytosis / physiology Endoplasmic Reticulum / metabolism Golgi Apparatus / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Metamorphosis, Biological / physiology* Monomeric GTP-Binding Proteins / genetics Neuronal Plasticity / genetics* Neuronal Plasticity / physiology Sensory Receptor Cells / cytology Sensory Receptor Cells / metabolism* rab GTP-Binding Proteins / genetics
IF 5.763
Times Cited 2