RRC ID 57235
Author Kohyama-Koganeya A, Kurosawa M, Hirabayashi Y.
Title Loss of BOSS Causes Shortened Lifespan with Mitochondrial Dysfunction in Drosophila.
Journal PLoS One
Abstract Aging is a universal process that causes deterioration in biological functions of an organism over its lifetime. There are many risk factors that are thought to contribute to aging rate, with disruption of metabolic homeostasis being one of the main factors that accelerates aging. Previously, we identified a new function for the putative G-protein-coupled receptor, Bride of sevenless (BOSS), in energy metabolism. Since maintaining metabolic homeostasis is a critical factor in aging, we investigated whether BOSS plays a role in the aging process. Here, we show that BOSS affects lifespan regulation. boss null mutants exhibit shortened lifespans, and their locomotor performance and gut lipase activity-two age-sensitive markers-are diminished and similar to those of aged control flies. Reactive oxygen species (ROS) production is also elevated in boss null mutants, and their ROS defense system is impaired. The accumulation of protein adducts (advanced lipoxidation end products [ALEs] and advanced glycation end products [AGEs]) caused by oxidative stress are elevated in boss mutant flies. Furthermore, boss mutant flies are sensitive to oxidative stress challenges, leading to shortened lives under oxidative stress conditions. Expression of superoxide dismutase 2 (SOD2), which is located in mitochondria and normally regulates ROS removal, was decreased in boss mutant flies. Systemic overexpression of SOD2 rescued boss mutant phenotypes. Finally, we observed that mitochondrial mass was greater in boss mutant flies. These results suggest that BOSS affects lifespan by modulating the expression of a set of genes related to oxidative stress resistance and mitochondrial homeostasis.
Volume 12(1)
Pages e0169073
Published 2017-1-3
DOI 10.1371/journal.pone.0169073
PII PONE-D-16-16302
PMID 28045997
PMC PMC5207625
MeSH Aging / physiology Animals Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Energy Metabolism Eye Proteins / genetics Eye Proteins / physiology* Female Glycation End Products, Advanced / metabolism Homeostasis Longevity* Male Membrane Glycoproteins / genetics Membrane Glycoproteins / physiology* Mitochondria / metabolism* Mutation Oxidative Stress Reactive Oxygen Species / metabolism* Receptors, Peptide / genetics Receptors, Peptide / physiology* Superoxide Dismutase / metabolism
IF 2.776
Times Cited 6