RRC ID 57263
Author Rogers AK, Situ K, Perkins EM, Toth KF.
Title Zucchini-dependent piRNA processing is triggered by recruitment to the cytoplasmic processing machinery.
Journal Genes Dev
Abstract The piRNA pathway represses transposable elements in the gonads and thereby plays a vital role in protecting the integrity of germline genomes of animals. Mature piRNAs are processed from longer transcripts, piRNA precursors (pre-piRNAs). In Drosophila, processing of pre-piRNAs is initiated by piRNA-guided Slicer cleavage or the endonuclease Zucchini (Zuc). As Zuc does not have any sequence or structure preferences in vitro, it is not known how piRNA precursors are selected and channeled into the Zuc-dependent processing pathway. We show that a heterologous RNA that lacks complementary piRNAs is processed into piRNAs upon recruitment of several piRNA pathway factors. This processing requires Zuc and the helicase Armitage (Armi). Aubergine (Aub), Argonaute 3 (Ago3), and components of the nuclear RDC complex, which are required for normal piRNA biogenesis in germ cells, are dispensable. Our approach allows discrimination of proteins involved in the transcription and export of piRNA precursors from components required for the cytoplasmic processing steps. piRNA processing correlates with localization of the substrate RNA to nuage, a distinct membraneless cytoplasmic compartment, which surrounds the nucleus of germ cells, suggesting that sequestration of RNA to this subcellular compartment is both necessary and sufficient for selecting piRNA biogenesis substrates.
Volume 31(18)
Pages 1858-1869
Published 2017-9-15
DOI 10.1101/gad.303214.117
PII gad.303214.117
PMID 29021243
PMC PMC5695087
MeSH Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Cytoplasm / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Endoribonucleases / genetics Endoribonucleases / metabolism* Female Germ Cells / metabolism Ovary / metabolism Peptide Initiation Factors / genetics Peptide Initiation Factors / metabolism RNA Helicases / genetics RNA Helicases / metabolism* RNA Precursors / metabolism* RNA Processing, Post-Transcriptional* RNA, Small Interfering / biosynthesis*
IF 8.99
Times Cited 16