論文 - 詳細
| RRC ID | 57348 |
|---|---|
| 著者 | Sanchez-Corrales YE, Blanchard GB, Röper K. |
| タイトル | Radially patterned cell behaviours during tube budding from an epithelium. |
| ジャーナル | Elife |
| Abstract |
The budding of tubular organs from flat epithelial sheets is a vital morphogenetic process. Cell behaviours that drive such processes are only starting to be unraveled. Using live-imaging and novel morphometric methods, we show that in addition to apical constriction, radially oriented directional intercalation of cells plays a major contribution to early stages of invagination of the salivary gland tube in the Drosophila embryo. Extending analyses in 3D, we find that near the pit of invagination, isotropic apical constriction leads to strong cell-wedging. Further from the pit cells interleave circumferentially, suggesting apically driven behaviours. Supporting this, junctional myosin is enriched in, and neighbour exchanges are biased towards the circumferential orientation. In a mutant failing pit specification, neither are biased due to an inactive pit. Thus, tube budding involves radially patterned pools of apical myosin, medial as well as junctional, and radially patterned 3D-cell behaviours, with a close mechanical interplay between invagination and intercalation. |
| 巻・号 | 7 |
| 公開日 | 2018-7-17 |
| DOI | 10.7554/eLife.35717 |
| PII | 35717 |
| PMID | 30015616 |
| PMC | PMC6089598 |
| MeSH | Animals Cell Polarity Cell Shape Drosophila melanogaster / cytology Drosophila melanogaster / embryology Embryo, Nonmammalian Epithelial Cells / cytology* Epithelium / embryology* Epithelium / metabolism Fluorescence Imaging, Three-Dimensional Morphogenesis* Mutation / genetics Myosin Type II / metabolism Salivary Glands / embryology Time-Lapse Imaging |
| IF | 7.551 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 126 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109822 |