Reference - Detail
|Author||Sanchez-Corrales YE, Blanchard GB, Röper K.|
|Title||Radially patterned cell behaviours during tube budding from an epithelium.|
embryo. Extending analyses in 3D, we find that near the pit of invagination, isotropic apical constriction leads to strong cell-wedging. Further from the pit cells interleave circumferentially, suggesting apically driven behaviours. Supporting this, junctional myosin is enriched in, and neighbour exchanges are biased towards the circumferential orientation. In a mutant failing pit specification, neither are biased due to an inactive pit. Thus, tube budding involves radially patterned pools of apical myosin, medial as well as junctional, and radially patterned 3D-cell behaviours, with a close mechanical interplay between invagination and intercalation.
|MeSH||Animals Cell Polarity Cell Shape Drosophila melanogaster / cytology Drosophila melanogaster / embryology Embryo, Nonmammalian Epithelial Cells / cytology* Epithelium / embryology* Epithelium / metabolism Fluorescence Imaging, Three-Dimensional Morphogenesis* Mutation / genetics Myosin Type II / metabolism Salivary Glands / embryology Time-Lapse Imaging|