| RRC ID |
57356
|
| 著者 |
Li S, Koe CT, Tay ST, Tan ALK, Zhang S, Zhang Y, Tan P, Sung WK, Wang H.
|
| タイトル |
An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins.
|
| ジャーナル |
Nat Commun
|
| Abstract |
The switch between quiescence and proliferation is central for neurogenesis and its alteration is linked to neurodevelopmental disorders such as microcephaly. However, intrinsic mechanisms that reactivate Drosophila larval neural stem cells (NSCs) to exit from quiescence are not well established. Here we show that the spindle matrix complex containing Chromator (Chro) functions as a key intrinsic regulator of NSC reactivation downstream of extrinsic insulin/insulin-like growth factor signalling. Chro also prevents NSCs from re-entering quiescence at later stages. NSC-specific in vivo profiling has identified many downstream targets of Chro, including a temporal transcription factor Grainy head (Grh) and a neural stem cell quiescence-inducing factor Prospero (Pros). We show that spindle matrix proteins promote the expression of Grh and repress that of Pros in NSCs to govern their reactivation. Our data demonstrate that nuclear Chro critically regulates gene expression in NSCs at the transition from quiescence to proliferation.The spindle matrix proteins, including Chro, are known to regulate mitotic spindle assembly in the cytoplasm. Here the authors show that in Drosophila larval brain, Chro promotes neural stem cell (NSC) reactivation and prevents activated NSCs from entering quiescence, and that Chro carries out such a role by regulating the expression of key transcription factors in the nucleus.
|
| 巻・号 |
8(1)
|
| ページ |
122
|
| 公開日 |
2017-7-25
|
| DOI |
10.1038/s41467-017-00172-9
|
| PII |
10.1038/s41467-017-00172-9
|
| PMID |
28744001
|
| PMC |
PMC5526931
|
| MeSH |
Animals
Animals, Genetically Modified
Blotting, Western
Cell Cycle Proteins
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Drosophila Proteins / genetics*
Drosophila Proteins / metabolism
Drosophila melanogaster / cytology
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism
Gene Expression Profiling / methods
Larva / cytology
Larva / genetics
Larva / metabolism
Microscopy, Confocal
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Neural Stem Cells / metabolism*
Nuclear Matrix-Associated Proteins / genetics*
Nuclear Matrix-Associated Proteins / metabolism
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Phosphoproteins / genetics*
Phosphoproteins / metabolism
RNA Interference
Transcription Factors / genetics
Transcription Factors / metabolism
|
| IF |
11.878
|
| 引用数 |
8
|
| リソース情報 |
| ショウジョウバエ |
DGRC#111163 |
