RRC ID 57375
Author Sereti KI, Oikonomopoulos A, Unno K, Liao R.
Title Methods to study the proliferation and differentiation of cardiac side population (CSP) cells.
Journal Methods Mol Biol
Abstract Investigation of cardiac progenitor cell proliferation and differentiation is essential for both the basic understanding of progenitor cell biology as well as the development of cellular therapeutics for tissue regeneration. Herein, we describe techniques used for the analysis of CSP cell proliferation, cell cycle status, and cardiomyogenic differentiation.
Volume 1036
Pages 95-106
Published 2013-1-1
DOI 10.1007/978-1-62703-511-8_9
PMID 23807790
MeSH Animals Animals, Newborn Cell Cycle Cell Differentiation* Cell Proliferation* Cell Separation / methods* Cells, Cultured Flow Cytometry Myocytes, Cardiac / cytology* Myocytes, Cardiac / metabolism Rats Side-Population Cells / cytology* Side-Population Cells / metabolism Stem Cells / cytology* Stem Cells / metabolism
DNA material mKO2-hCdt1(30/120) / pCSII-EF (RDB15267) mAG-hGeminin(1/110) / pCSII-EF (RDB15268) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)