論文 - 詳細
| RRC ID | 57789 |
|---|---|
| 著者 | Matsubara H, Tanaka R, Tateishi T, Yoshida H, Yamaguchi M, Kataoka T. |
| タイトル | The human Bcl-2 family member Bcl-rambo and voltage-dependent anion channels manifest a genetic interaction in Drosophila and cooperatively promote the activation of effector caspases in human cultured cells. |
| ジャーナル | Exp Cell Res |
| Abstract |
We previously reported that the Bcl-2 family member human Bcl-rambo, also known as BCL2L13, induces apoptosis in human embryonic kidney 293T cells. Mouse Bcl-rambo has recently been reported to mediate mitochondrial fragmentation and mitophagy. In the present study, we showed that the transfection of human Bcl-rambo and its microtubule-associated protein light chain 3-interacting region motif mutant (W276A/I279A) caused mitochondrial fragmentation and the perinuclear accumulation of fragmented mitochondria in human lung adenocarcinoma A549 cells. In comprehensive screening using the Drosophila model in which human Bcl-rambo was ectopically expressed in eye imaginal discs, voltage-dependent anion channels (VDAC), also known as mitochondrial porin, were found to manifest a genetic interaction with human Bcl-rambo. In addition to human adenine nucleotide translocase (ANT) 1 and ANT2, the human Bcl-rambo protein bound to human VDAC1, albeit to a lesser extent than ANT2. Moreover, human VDAC1 and human VDAC2 in particular promoted the activation of effector caspases only when they were co-expressed with human Bcl-rambo in 293T cells. Bcl-rambo induced the perinuclear accumulation of fragmented mitochondria by the knockdown of VDAC1, VDAC2, and VDAC3 in A549 cells. Thus, the present study revealed that human Bcl-rambo and VDAC cooperatively promote the activation of effector caspases in human cultured cells. |
| 巻・号 | 381(2) |
| ページ | 223-234 |
| 公開日 | 2019-8-15 |
| DOI | 10.1016/j.yexcr.2019.05.015 |
| PII | S0014-4827(19)30256-3 |
| PMID | 31102594 |
| MeSH | A549 Cells Animals Animals, Genetically Modified Caspases, Effector / metabolism* Cells, Cultured Drosophila / genetics* Enzyme Activation / genetics Epistasis, Genetic / physiology* HEK293 Cells Humans Mice Mitochondrial Membrane Transport Proteins / genetics Proto-Oncogene Proteins c-bcl-2 / genetics* Voltage-Dependent Anion Channel 1 / genetics Voltage-Dependent Anion Channel 2 / genetics Voltage-Dependent Anion Channels / genetics* |
| IF | 3.383 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |