RRC ID 57825
Author Tang JY, Li LJ, Ou-Yang F, Wang CL, Shu CW, Wu KH, Wang HR, Yen CH, Cheng YB, Chang HW.
Title Ethyl Acetate Extract of Nepenthes ventricosa x maxima Exerts Preferential Killing to Oral Cancer Cells.
Journal DNA Cell Biol.
Abstract Nepenthes plants are a folk medicine in many Southeast Asia countries for curing diseases but its anticancer effect is rarely investigated. The objectives of this study were to investigate the antioral cancer ability of ethyl acetate extract of Nepenthes ventricosa x maxima (EANV). The preferential killing ability of EANV was determined by MTS-based cell viability assays. The bioactive effects were further screened by flow cytometry for apoptosis, oxidative stress, and DNA damage. At 24 h treatment, EANV dose dependently decreased six types of oral cancer cells, but the normal oral cells (HGF-1) kept a 90% viability. EANV also showed chronic antiproliferative effects and inhibited 3D sphere formation ability of oral cancer cells. Ca9-22 and CAL 27 oral cancer cells with high response to EANV increased subG1 populations and enhanced Annexin V- and pancaspase-detected apoptosis in these cells. EANV also induced the generation of reactive oxygen species (ROS) and mitochondrial superoxide and the dysfunction of mitochondrial membrane potential. Moreover, the oxidative DNA damage level such as 8-oxo-2'deoxyguanosine was increased in EANV-treated oral cancer cells. Taken together, EANV has a preferential killing effect against oral cancer cells associated with oxidative stress, apoptosis, and DNA damage, suggesting EANV as a potential antioral cancer agent.
Volume 38(8)
Pages 763-772
Published 2019-8
DOI 10.1089/dna.2018.4436
PMID 31170002
MeSH Acetates / chemistry Antineoplastic Agents, Phytogenic / chemistry Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis / drug effects Caryophyllales / chemistry* Cell Line, Tumor DNA Damage / drug effects Drug Screening Assays, Antitumor Humans Membrane Potential, Mitochondrial / drug effects Mouth Neoplasms / drug therapy* Mouth Neoplasms / pathology Oxidative Stress / drug effects Plant Extracts / chemistry Plant Extracts / pharmacology* Reactive Oxygen Species / metabolism Spectrometry, Mass, Electrospray Ionization
IF 2.918
Times Cited 1
Human and Animal Cells Ca9-22(RCB1976) HSC-3(RCB1975)