RRC ID 57879
著者 Ser-Od T, Al-Wahabi A, Inoue K, Nakajima K, Matsuzaka K, Inoue T.
タイトル Effect of EDTA-treated dentin on the differentiation of mouse iPS cells into osteogenic/odontogenic lineages in vitro and in vivo.
ジャーナル Dent Mater J
Abstract To investigate the effect of EDTA-treated dentin on the differentiation of mouse induced pluripotent stem (iPS) cells. Dentin discs were prepared from bovine incisors and treated with 17% EDTA. Embryoid bodies (EBs) formed from mouse iPS cells were seeded on the dentin discs for the experiment. The roughness of the EDTA-treated dentin surface, Sa and Sdr, was higher and collagen fibrillike structures were observed by the scanning electron microscopy (SEM) in vitro. In RT-PCR, the mRNA levels of the osteoblast markers Bsp and Ocn were significantly higher in the experimental group. Expression of the DMP1, DSP, and BSP proteins were more notable in the experimental group by immunofluorescence (ICF) study. In vivo study, cartilage and bone-like tissue were observed adjacent to the EDTA-treated dentin. The study demonstrates that the dentin treated with 17% EDTA induces mouse iPS cells to differentiate into the osteo/odontogenesis.
巻・号 38(5)
ページ 830-838
公開日 2019-10-2
DOI 10.4012/dmj.2018-161
PMID 31341145
MeSH Animals Cattle Cell Differentiation Cells, Cultured Dentin Edetic Acid Induced Pluripotent Stem Cells* Mice Odontogenesis
IF 1.359
引用数 0
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-178B-5(APS0002)