論文 - 詳細
| RRC ID | 57884 |
|---|---|
| 著者 | Goto T, Homma H, Kaida A, Miura M. |
| タイトル | WEE1 inhibition enhances sensitivity to hypoxia/reoxygenation in HeLa cells. |
| ジャーナル | J Radiat Res |
| Abstract |
Hypoxia/reoxygenation (H/R) treatment reportedly induces DNA damage response (DDR), including DNA double-strand break (DSB) repair and G2 arrest, resulting in reduction of clonogenic survival. Because WEE1 plays a key role in the G2/M checkpoint along with CHK1/2, we investigated the effect of WEE1 inhibition on H/R-induced DDR using HeLa cells. The H/R treatment combined with WEE1 inhibitor abrogated G2 arrest, subsequently leading to the cells entering the M phase, and finally resulting in mitotic catastrophe after prolonged mitosis. Colony-forming assay showed an enhanced decrease in the surviving fraction and the focus formation of BRCA1 was significantly reduced. We demonstrate for the first time that WEE1 inhibition enhances H/R-induced cell death accompanied by mitotic catastrophe and that the process may be mediated by homologous recombination. |
| 巻・号 | 60(5) |
| ページ | 709-713 |
| 公開日 | 2019-10-23 |
| DOI | 10.1093/jrr/rrz045 |
| PII | 5539284 |
| PMID | 31347653 |
| PMC | PMC6805980 |
| MeSH | BRCA1 Protein / metabolism Cell Cycle Proteins / antagonists & inhibitors* Cell Cycle Proteins / metabolism Cell Hypoxia / drug effects Cell Survival / drug effects Clone Cells G2 Phase Cell Cycle Checkpoints / drug effects HeLa Cells Humans Kinetics Mitosis / drug effects Oxygen / pharmacology* Protein-Tyrosine Kinases / antagonists & inhibitors* Protein-Tyrosine Kinases / metabolism Pyrazoles / pharmacology Pyrimidinones / pharmacology |
| IF | 2.014 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa/Fucci(RCB2812) |