| RRC ID |
58008
|
| Author |
Li Y, Liu YD, Zhou XY, Chen SL, Chen X, Zhe J, Zhang J, Zhang QY, Chen YX.
|
| Title |
MiR-29a regulates the proliferation, aromatase expression, and estradiol biosynthesis of human granulosa cells in polycystic ovary syndrome.
|
| Journal |
Mol Cell Endocrinol
|
| Abstract |
Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility in reproductive-aged women; however, its etiology remains poorly understood. This study aimed to reveal the role of miR-29a in PCOS. MiR-29a levels were measured in the granulosa cells (GCs) of forty-seven PCOS patients and forty-seven controls. A receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic value of miR-29a in non-hyperandrogenism PCOS. MiR-29a was overexpressed in KGN and COV434 cells to examine its roles in proliferation, cell-cycle progression, and steroidogenesis. MiR-29a was significantly down-regulated in PCOS patients, and associated with an increased antral follicle count. The ROC curve showed a major separation between PCOS patients and controls. MiR-29a overexpression in KGN and COV434 cells inhibited cell proliferation, arrested cell-cycle progression, and decreased aromatase expression and estradiol production. These findings suggest that miR-29a is involved in GC proliferation and steroidogenesis, providing insights into PCOS pathogenesis.
|
| Volume |
498
|
| Pages |
110540
|
| Published |
2019-12-1
|
| DOI |
10.1016/j.mce.2019.110540
|
| PII |
S0303-7207(19)30242-4
|
| PMID |
31421163
|
| MeSH |
Adult
Apoptosis
Aromatase / genetics
Aromatase / metabolism*
Biomarkers / metabolism*
Case-Control Studies
Cell Cycle
Cell Proliferation*
Cells, Cultured
Estradiol / metabolism*
Female
Follow-Up Studies
Granulosa Cells / metabolism
Granulosa Cells / pathology*
Humans
MicroRNAs / genetics*
Polycystic Ovary Syndrome / genetics
Polycystic Ovary Syndrome / metabolism
Polycystic Ovary Syndrome / pathology*
Prognosis
|
| IF |
3.871
|
| Times Cited |
2
|
| Resource |
| Human and Animal Cells |
KGN(RCB1154) |
