| RRC ID |
58060
|
| 著者 |
Tateyama H, Murase Y, Higuchi H, Inasaka Y, Kaneoka H, Iijima S, Nishijima KI.
|
| タイトル |
Siglec-F is induced by granulocyte-macrophage colony-stimulating factor and enhances interleukin-4-induced expression of arginase-1 in mouse macrophages.
|
| ジャーナル |
Immunology
|
| Abstract |
Siglecs are cell surface lectins that recognize sialic acids and are primarily expressed in hematopoietic cells. Previous studies showed that some Siglecs regulate macrophage function. In the present study, we examined the induction and putative roles of mouse Siglec-F in bone-marrow-derived macrophages in mice. A quantitative RT-PCR analysis showed that the basal expression of Siglec-F was weak in bone-marrow-derived macrophages differentiated by macrophage colony-stimulating factor. However, a 24-hr stimulation with granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced Siglec-F expression. GM-CSF also enhanced Siglec-F expression in thioglycollate-induced peritoneal macrophages. The inhibition of signal transducer and activator of transcription 5 (STAT5), but not that of phosphoinositide 3-kinase or mitogen-activated protein kinase kinase, significantly reduced the induction of Siglec-F. Interleukin-3, which uses a common β-chain shared with the GM-CSF receptor to stimulate the STAT5 pathway, also enhanced Siglec-F expression. The knockdown of Siglec-F by a specific small interfering RNA enhanced GM-CSF-induced STAT5 phosphorylation, suggesting that Siglec-F down-regulates its own expression upon prolonged GM-CSF stimulation. Furthermore, the knockdown of Siglec-F reduced the STAT6 phosphorylation and expression of arginase-1 in interleukin-4-stimulated macrophages. These results suggest that Siglec-F fine-tunes the immune responses of macrophages.
|
| 巻・号 |
158(4)
|
| ページ |
340-352
|
| 公開日 |
2019-12-1
|
| DOI |
10.1111/imm.13121
|
| PMID |
31520477
|
| PMC |
PMC6856935
|
| MeSH |
Animals
Antigens, Differentiation, Myelomonocytic / genetics
Antigens, Differentiation, Myelomonocytic / metabolism*
Arginase / genetics
Arginase / metabolism*
Cells, Cultured
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism*
Interleukin-4 / genetics
Interleukin-4 / metabolism*
Macrophages / immunology*
Male
Mice
Mice, Inbred C57BL
Phosphorylation
RNA, Small Interfering / genetics
STAT5 Transcription Factor / metabolism
STAT6 Transcription Factor / metabolism
Sialic Acid Binding Immunoglobulin-like Lectins
Up-Regulation
|
| IF |
5.016
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
L929 |
