Reference - Detail
| RRC ID | 581 |
|---|---|
| Author | Shimizu R, Taguchi F, Marutani M, Mukaihara T, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y. |
| Title | The DeltafliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cells. |
| Journal | Mol Genet Genomics |
| Abstract |
To investigate the role of flagella and monomer flagellin in the interaction between Pseudomonas syringae pv. tabaci and plants, non-polar fliC and fliD mutants were produced. The ORFs for fliC and fliD are deleted in the DeltafliC and DeltafliD mutants, respectively. Both mutants lost all flagella and were non-motile. The DeltafliC mutant did not produce flagellin, whereas the DeltafliD mutant, which lacks the HAP2 protein, secreted large amounts of monomer flagellin into the culture medium. Inoculation of non-host tomato leaves with wild-type P. syringae pv. tabaci or the DeltafliD mutant induced a hypersensitive reaction (HR), whereas the DeltafliC mutant propagated and caused characteristic symptom-like changes. In tomato cells in suspension culture, wild-type P. syringae pv. tabaci induced slight, visible HR-like changes. The DeltafliC mutant did not induce HR, but the DeltafliD mutant induced a remarkably strong HR. Expression of the hsr203J gene was rapidly and strongly induced by inoculation with the DeltafliD mutant, compared to inoculation with wild-type P. syringae pv. tabaci. Furthermore, introduction of the fliC gene into the DeltafliC mutant restored motility and HR-inducing ability in tomato. These results, together with our previous study, suggest that the flagellin monomer of pv. tabaci acts as a strong elicitor to induce HR-associated cell death in non-host tomato cells. |
| Volume | 269(1) |
| Pages | 21-30 |
| Published | 2003-4-1 |
| DOI | 10.1007/s00438-003-0817-3 |
| PMID | 12715150 |
| MeSH | Bacterial Outer Membrane Proteins / immunology* Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* Base Sequence Cells, Cultured Culture Media / analysis Flagella / metabolism Flagellin / chemistry Flagellin / immunology Flagellin / metabolism* Gene Deletion Gene Expression Genes, Bacterial Lycopersicon esculentum / cytology Lycopersicon esculentum / microbiology* Molecular Sequence Data Open Reading Frames Plant Diseases / microbiology Pseudomonas / genetics Pseudomonas / metabolism* Recombinant Proteins / metabolism |
| IF | 2.797 |
| Times Cited | 61 |
| WOS Category | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00012 |
| Prokaryotes E. coli | |