RRC ID 58227
Author Ogura T, Yokoyama T, Fujisawa H, Kurashima Y, Esumi H.
Title Structural diversity of neuronal nitric oxide synthase mRNA in the nervous system.
Journal Biochem Biophys Res Commun
Abstract Nitric oxide (NO), generated from L-arginine by an enzymatic reaction of a NO synthase (NOS; EC 1.14.23), is a recently identified biological mediator suggested to be involved in a wide variety of biological processes. In the present work, we isolated and sequenced the entire region of cDNAs encoding mouse neuronal NOS (n-NOS) and demonstrated structural diversity of n-NOS mRNA in the nervous system. Sequence determination revealed a novel mRNA with an inframe deletion in the middle of the n-NOS cDNA. Structural analysis of the corresponding part of the n-NOS gene indicated that the deletion corresponded exactly to two exons. These findings suggest that the variant n-NOS is formed by alternative splicing. Both n-NOSs were found in almost all parts of the nervous system, the expression level of the novel variant being about one twentieth of that of the known n-NOS. Generation of diversity of n-NOS is probably related to diversity of its biological functions.
Volume 193(3)
Pages 1014-22
Published 1993-6-30
DOI 10.1006/bbrc.1993.1726
PII S0006-291X(83)71726-2
PMID 7686743
MeSH Amino Acid Oxidoreductases / biosynthesis* Amino Acid Oxidoreductases / genetics* Amino Acid Sequence Animals Base Sequence Brain / enzymology* Cloning, Molecular Gene Library Genetic Variation* Mice Mice, Inbred BALB C Molecular Sequence Data Neurons / enzymology* Nitric Oxide Synthase Oligodeoxyribonucleotides Organ Specificity Poly A / genetics Poly A / isolation & purification Polymerase Chain Reaction RNA / genetics RNA / isolation & purification RNA, Messenger / genetics* RNA, Messenger / metabolism* Spinal Cord / enzymology*
IF 2.985
Times Cited 113
DNA material Mouse Neuronal NOS cDNA (RDB01386)