論文 - 詳細
| RRC ID | 58234 |
|---|---|
| 著者 | Oh S, Kawasaki I, Park JH, Shim YH. |
| タイトル | cdc-25.4, a Caenorhabditis elegans Ortholog of cdc25, Is Required for Male Mating Behavior. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
Cell division cycle 25 (cdc25) is an evolutionarily conserved phosphatase that promotes cell cycle progression. Among the four cdc25 orthologs in Caenorhabditis elegans, we found that cdc-25.4 mutant males failed to produce outcrossed progeny. This was not caused by defects in sperm development, but by defects in male mating behavior. The cdc-25.4 mutant males showed various defects during male mating, including contact response, backing, turning, and vulva location. Aberrant turning behavior was the most prominent defect in the cdc-25.4 mutant males. We also found that cdc-25.4 is expressed in many neuronal cells throughout development. The turning defect in cdc-25.4 mutant males was recovered by cdc-25.4 transgenic expression in neuronal cells, suggesting that cdc-25.4 functions in neurons for male mating. However, the neuronal morphology of cdc-25.4 mutant males appeared to be normal, as examined with several neuronal markers. Also, RNAi depletion of wee-1.3, a C. elegans ortholog of Wee1/Myt1 kinase, failed to suppress the mating defects of cdc-25.4 mutant males. These findings suggest that, for successful male mating, cdc-25.4 does not target cell cycles that are required for neuronal differentiation and development. Rather, cdc-25.4 likely regulates noncanonical substrates in neuronal cells. |
| 巻・号 | 6(12) |
| ページ | 4127-4138 |
| 公開日 | 2016-12-7 |
| DOI | 10.1534/g3.116.036129 |
| PII | g3.116.036129 |
| PMID | 27770028 |
| PMC | PMC5144981 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Cell Cycle Proteins / genetics* Fertility / genetics Gene Expression Genetic Association Studies* Germ Cells / metabolism Male Mutation Neurons / metabolism Sexual Behavior, Animal* Spermatozoa / metabolism Transgenes |
| IF | 2.781 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4088 |